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（ 母液浓度为 40 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们客服联系。| 中文名称: | 土壤基因组DNA提取试剂盒 一键复制产品信息 | ||||
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产品简介：
本产品综合MP公司的土壤DNA提取试剂盒产量高和Mobio公司纯度高的优点研制而成，采取MP公司完全一致的研磨管（3种研磨珠混合）方案可以处理更大的样品，可以直接使用MP研磨管一样的珠磨仪器和参数。独有的去腐殖酸技术可以完美去除各种腐殖酸和杂质的影响，提取的DNA纯度高，可以直接用于下游PCR反应。
操作步骤：
Before the first use, add the indicated amount of ethanol into PQ solution bottle, Buffer WB bottle, mix well, and mark the bottle with a check.
1：Add up to 500 mg of soil sample to a Bead Tube.
2：Add 980 μl Sodium Phosphate Buffer to sample in Bead Tube.Gentle votex to mix. Add 120 μl MT Buffer.
Note: Check MT Buffer. If MT Buffer is precipitated, heat solution to 60°C until dissolved before use.
3：Homogenize in the FastPrep® Instrument for 40 seconds at a speed setting of 6.0.
4：Centrifuge at 12,000 x g for 5minutes to pellet debris.
5：Transfer supernatant to a clean 2.0 ml centrifuge tube. Add 250μl PPS Solution and mix by shaking the tube by hand 10 times.Incubate at 4°C for 5 minutes.
6：Centrifuge tubes at 10,000 x g for 3 minute at room temperature.Avoiding pellet, transfer up to, but no more than, 900 μl of supernatant to a clean 2 ml centrifuge tube.
7：Add 300 μl of IRS Solution(1/3 volume) and vortex briefly. Incubate at 4°C for 5 minutes.
8：Centrifuge tubes at 10,000 x g for 1 minute at room temperature. Avoiding pellet, transfer the supernatant into a clean 5 ml centrifuge tube.
9：Add 1.5 volumes of PQ Solution to the cleared supernatant and mix by pipetting.
Example: To 1100 μl lysate add 1650 μl PQ Solution. Reduce the amount of PQ Solution accordingly if less supernatant is recovered. A precipitate may form after the addition of ethanol but this will not affect the procedure.
Note: Ensure ethanol has been added to PQ Solution.
Note: It is important to pipet PQ Solution directly onto the cleared supernatant and to mix immediately.
10：Load approximately 700 μl mixture onto Spin Filter(sitting in collection tube) and centrifuge at 10,000 x g for 1 minute at room temperature. Discard flow through. Load another 700 μl and repeat untill all remaining mixture is loaded on Spin Filter.
Note: A total of 4-5 loads for each sample processed may be required.
11：Add 600 μl of Buffer WB to Spin Filter and centrifuge at 10,000 x g for 30 seconds at room temperature. Discard flow through. Repeat Step 11 with another 600 μl Buffer WB.
Note: Ensure ethanol is added to Buffer WB.
12：CentrifugeSpinFilterat13,000xgfor2minuteatroomtemperature to dry the Spin Filter.
13：Carefully place Spin Filter in clean 1.5 ml centrifuge tube. Avoid splashing any Buffer WB onto Spin Filter. Add 100 μl of Elution Buffer (Optional: pre-warm the water to 70–90C will increase the DNA yield) to the center of the column membrane. Incubate at room temperature for 3-5 min, and centrifuge at 13,000 x g for 1 min to elute the DNA.
Note: Use smaller volume(minimum 30μl) of Elution Buffer will obtain higher concentration.
Optional: Put eluate back to the Spin Column to repeat elution once. This increases concentration of DNA about 10-15%.
1：Add up to 500 mg of soil sample to a Bead Tube.
2：Add 980 μl Sodium Phosphate Buffer to sample in Bead Tube.Gentle votex to mix. Add 120 μl MT Buffer.
Note: Check MT Buffer. If MT Buffer is precipitated, heat solution to 60°C until dissolved before use.
3：Homogenize in the FastPrep® Instrument for 40 seconds at a speed setting of 6.0.
4：Centrifuge at 12,000 x g for 5minutes to pellet debris.
5：Transfer supernatant to a clean 2.0 ml centrifuge tube. Add 250μl PPS Solution and mix by shaking the tube by hand 10 times.Incubate at 4°C for 5 minutes.
6：Centrifuge tubes at 10,000 x g for 3 minute at room temperature.Avoiding pellet, transfer up to, but no more than, 900 μl of supernatant to a clean 2 ml centrifuge tube.
7：Add 300 μl of IRS Solution(1/3 volume) and vortex briefly. Incubate at 4°C for 5 minutes.
8：Centrifuge tubes at 10,000 x g for 1 minute at room temperature. Avoiding pellet, transfer the supernatant into a clean 5 ml centrifuge tube.
9：Add 1.5 volumes of PQ Solution to the cleared supernatant and mix by pipetting.
Example: To 1100 μl lysate add 1650 μl PQ Solution. Reduce the amount of PQ Solution accordingly if less supernatant is recovered. A precipitate may form after the addition of ethanol but this will not affect the procedure.
Note: Ensure ethanol has been added to PQ Solution.
Note: It is important to pipet PQ Solution directly onto the cleared supernatant and to mix immediately.
10：Load approximately 700 μl mixture onto Spin Filter(sitting in collection tube) and centrifuge at 10,000 x g for 1 minute at room temperature. Discard flow through. Load another 700 μl and repeat untill all remaining mixture is loaded on Spin Filter.
Note: A total of 4-5 loads for each sample processed may be required.
11：Add 600 μl of Buffer WB to Spin Filter and centrifuge at 10,000 x g for 30 seconds at room temperature. Discard flow through. Repeat Step 11 with another 600 μl Buffer WB.
Note: Ensure ethanol is added to Buffer WB.
12：CentrifugeSpinFilterat13,000xgfor2minuteatroomtemperature to dry the Spin Filter.
13：Carefully place Spin Filter in clean 1.5 ml centrifuge tube. Avoid splashing any Buffer WB onto Spin Filter. Add 100 μl of Elution Buffer (Optional: pre-warm the water to 70–90C will increase the DNA yield) to the center of the column membrane. Incubate at room temperature for 3-5 min, and centrifuge at 13,000 x g for 1 min to elute the DNA.
Note: Use smaller volume(minimum 30μl) of Elution Buffer will obtain higher concentration.
Optional: Put eluate back to the Spin Column to repeat elution once. This increases concentration of DNA about 10-15%.
组分：
| 组分名称 | 50T | 储存 |
| Bead Tube | 50EA | 室温 |
| Sodium Phosphate Buffer | 50mL | 室温 |
| MT Buffer | 6mL | 室温 |
| PPS Solution | 13mL | 室温 |
| IRS Solution | 15mL | 室温 |
| PQ Solution | 35mL(首次使用前添加两倍体积的乙醇) | 室温 |
| Buffer WB | 13mL(首次使用前添加四倍体积的乙醇) | 室温 |
| Elution Buffer | 15mL | 室温 |
| DNA Bind Columns | 50EA | 室温 |
保存条件：
室温运输，按说明书保存 。
自备试剂：
无水乙醇(E20003)
UN码：
HazardClass：
危害声明：
安全说明：
搜索质检报告(COA)
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
(2).jpg)
体内配方的制备方法: 取 50μLDMSO 母液，添加 300 μLPEG300 混匀澄清，再加 50μLTween80, 混匀澄清，再加 600μLSaline/PBS/ddH2O 混匀澄清
方案所需的各种助溶剂如： DMSO ， PEG300 / PEG400 ， Tween 80 ， SBE-β-CD ， 玉米油 等， 均可点击跳转或在网站搜索购买。
母液，添加 300 μLPEG300 混匀澄清，再加 50μLTween80, 混匀澄清，再加 600μLSaline/PBS/ddH2O 混匀澄清方案所需的各种助溶剂如： DMSO ， PEG300 / PEG400 ， Tween 80 ， SBE-β-CD ， 玉米油 等， 均可点击跳转或在网站搜索购买。
以上为“动物实验计算换算器”的使用方法举例，并不是具体某个试剂的配制，请根据您的实验动物和给药方式选择适当的溶解方案。
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH2O，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
剂量转换
对于不同动物的给药剂量换算，您也可以参考 更多
