C75 - CAS:218137-86-1 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	C75 促销
英文名称:	C75
别名:	C75 4-Methylene-2-octyl-5-oxotetrahydrofuran-3-carboxylic acid
CAS No:	218137-86-1	分子式:	C₁₄H₂₂O₄	分子量:	254.32
CAS No:	218137-86-1
分子式:	C₁₄H₂₂O₄
分子量:	254.32

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF) 点击下载技术规格说明书(PDF)

C11234 C75 ≥98% (psaitong)

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包装规格：

5mg 10mg 50mg in glass bottle

溶解性：

溶于DMSO(5mg/ml）

产品描述：

基本信息

产品编号：	C11234
产品名称：	C75
CAS:	218137-86-1	储存条件	粉末	-20℃	四年

分子式：	C14H22O4		溶于液体	-80℃	两年
分子量	254.32			-20℃	一个月
化学名：	4-Methylene-2-octyl-5-oxotetrahydrofuran-3-carboxylic acid
Solubility (25°C)：
体外:	DMSO	51mg/mL (200.53mM)
	Ethanol	51mg/mL (200.53mM)
	Water	Insoluble
体内(现配现用):	1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.5mg/mL (9.83mM); Clear solution
	此⽅案可获得 ≥ 2.5mg/mL (9.83mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。
	2.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 2.5mg/mL (9.83mM); Clear solution
	此⽅案可获得 ≥ 2.5mg/mL (9.83mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。
	3.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2.5mg/mL (9.83mM); Clear solution
	此⽅案可获得 ≥ 2.5mg/mL (9.83mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	3.9321mL	19.6603mL	39.3205mL
5mM	0.7864mL	3.9321mL	7.8641mL
10mM	0.3932mL	1.9660mL	3.9321mL
50mM	0.0786mL	0.3932mL	0.7864mL

生物活性

产品描述	合成的脂肪酸合成酶(FASN)抑制剂。
靶点	FAS/FASN () 15.53μM
体外研究	C75 inhibits PC3 cell growht with an IC50 of 35μM at 24 h. C75 (10-50μM) also reduces the growth of LNCaP spheroids in a concentration-dependent manner with an IC50 of 50μM. (-)-C75 inhibits FAS activity and has a cytotoxic effect on tumor cell lines, without affecting food consumption. (+)-C75 inhibits CPT1 and its administration produces anorexia, suggesting that central inhibition of CPT1 is essential for the anorectic effect of C75. The differential activity of C75 enantiomers may lead to the development of potential new specific drugs for cancer and obesity
体内研究	C75 blocks fasting-induced c-Fos expression in the arcuate nucleus (Arc), lateral hypothalamic area (LHA), and paraventricular nucleus (PVN) 10–24 h after i.p. injection. Intraperitoneal administration of C75 at 30mg/kg body weight inhibits food intake of mice by ≥95% within 2 h after i.p. injection. C75-treated DIO mice has a 50% greater weight loss, and a 32.9% increased production of energy because of fatty acid oxidation. C75 treatment of rodent adipocytes and hepatocytes and human breast cancer cells increases fatty acid oxidation and ATP levels by increasing CPT-1 activity, even in the presence of elevated concentrations of malonyl-CoA

推荐实验方法(仅供参考)

Cell Assay	Cells are seeded in 96-well plates and incubated for 2 days to allow exponential phase growth. Cells are then ished twice with PBS and treated with C75. After 24 or 48 h incubation, MTT is added to a final concentration of 0.5mg/ml and cultures are incubated for 2h. Cells are then solubilized with DMSO before measuring absorbance at 570 nm. Cell growth is also measured, using MTT assay, every 24 h up to 96h

Animal Administration	Mice: C75 is administered either by i.p. (i.p.; 30mg/kg of body weight) or i.c.v. (10 μg in 3μL of RPMI medium 1640) injection. One, 11.5, and 24 h after i.p. injection, cumulative food intake is measured, mice are killed, brains are sectioned, and slices are subjected to immunohistochemical staining for c-Fos. All i.p. injections are given 1 h before the start of the dark cycle. For i.c.v. injection, mice are anesthetized with metofane and given 3μL of RPMI medium 1640 (control) or C75 in RPMI medium 1640 into the lateral ventricle with a calibrated 10-μl Hamilton syringe