β-Casomorphin, human - CAS:102029-74-3 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	β-Casomorphin, human 一键复制产品信息
英文名称:	β-Casomorphin, human
别名:	Tyr-Pro-Phe-Val-Glu-Pro-Ile
CAS No:	102029-74-3	分子式:	C₄₄H₆₁N₇O₁₁	分子量:	864
CAS No:	102029-74-3
分子式:	C₄₄H₆₁N₇O₁₁
分子量:	864

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C11537 β-Casomorphin, human (普西唐-psaitong)

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包装规格：

5mg 10mg 50mg in glass bottle

产品描述：

基本信息

产品编号：	C11537
产品名称：	β-Casomorphin, human
CAS:	102029-74-3	储存条件	粉末	-20℃	四年

分子式：	C44H61N7O11		溶于液体
分子量	864.00
化学名：	Tyr-Pro-Phe-Val-Glu-Pro-Ile

生物活性

产品描述

一种阿片样肽，为 opioid 受体激动剂。

靶点

Opioid receptor

体外研究

β-Casomorphin, human TFA induces contractions of the guinea-pig ileum myenteric plexus/longitudinal muscle preparation with an IC50 of 29μM.

β-Casomorphin, human TFA displaces 3H-spiperone from receptors in frontal cortex starting with 500nM concentrations

推荐实验方法(仅供参考)

Cell Assay

To study the effect of β-Casomorphin, human and DAMGO, cells are seeded in 12-well culture plates. Experiments are performed 3 (DHE cells) or 21 days (HT29-MTX cells) after confluency. Twenty-four hours before the studies, the culture medium is replaced by serum-free medium to starve the cells from serum and to eliminate any interference from extraneous proteins or hormones. The experimental protocol is then the following: the serum-free medium is removed, and the monolayer cultures of DHE or HT29-MTX are washed twice with PBS (37°C). Serum-free medium with or without βCasomorphin, human or DAMGO is added to the cells and incubated at 37°C for 30 min to 24 h in a humidified atmosphere. μ-Opioid receptor blockade is performed by preincubating the cells with cyprodime for 30 min before agonist addition. The supernatants are then collected, frozen, and stored at 20°C. Cells are processed with trypsin. The cell numbers per well are determined, and total RNA is isolated. All experiments are performed at least three times in triplicate

保存条件：

-20℃

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