SB225002 - CAS:182498-32-4 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	SB225002 一键复制产品信息
英文名称:	SB225002
别名:	N-(2-羟基-4-硝基苯基)-N'-(2-溴苯基)脲;1-(2-溴苯基)-3-(2-羟基-4-硝基苯基)脲 1-(2-bromophenyl)-3-(2-hydroxy-4-nitrophenyl)urea;N-(2-hydroxy-4-nitrophenyl)-N'-(2-bromophenyl)urea
CAS No:	182498-32-4	分子式:	C₁₃H₁₀BrN₃O₄	分子量:	352.14
CAS No:	182498-32-4
分子式:	C₁₃H₁₀BrN₃O₄
分子量:	352.14

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF)

S10856 SB225002 ≥98% (普西唐-psaitong)

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包装规格：

1g in glass bottle

溶解性：

溶于DMSO（≥100mg/mL）

产品描述：

基本信息

产品编号：	S10856
产品名称：	SB225002
CAS:	182498-32-4	储存条件	粉末	2-8℃(氮气保存)	四年
分子式：	C13H10BrN3O4		溶于液体	-80℃(氮气保存)	6个月
分子量：	352.14			-20℃(氮气保存)	1个月
化学名：	1-(2-bromophenyl)-3-(2-hydroxy-4-nitrophenyl)urea
Solubility (25°C)：
体外:	DMSO	70mg/mL (198.78mM)
	Ethanol	14mg/mL warmed with 50ºC water bath (39.75mM)
	Water	Insoluble
体内(现配现用):	2% DMSO+30% PEG 300+5% Tween 80+ddH2O	5mg/mL
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.8398mL	14.1989mL	28.3978mL
5mM	0.5680mL	2.8398mL	5.6796mL
10mM	0.2840mL	1.4199mL	2.8398mL
50mM	0.0568mL	0.2840mL	0.5680mL

生物活性

产品描述	一种有效的选择性 CXCR2 非肽拮抗剂，抑制 125I-IL-8 和 CXCR2 结合的 IC50 为 22nM。
靶点	125I-IL-8-CXCR2 22nM (IC50,in CHO cell membrane)
体外研究	SB225002 (SB 225002) is an antagonist of 125I-IL-8 binding to CXCR2 with an IC50=22nM.SB225002 shows>150-fold selectivity over CXCR1 and four other 7-TMRs tested.SB225002 is a potent antagonist of rabbit CXCR2,inhibiting rabbit PMN chemotaxis in response to optimal concentrations of human IL-8 or GROα (IC50 values of 30 and 70nM,respectively.In these cells (PMN, HL60,CXCR1-RBL-2H3), SB225002 produces a concentration-dependent inhibition of both IL-8- and GROαmediated calcium mobilization with IC50 values of 8 and 10nM,respectively.In 3ASubE cells stably transfected with CXCR2,SB 225002 dose-dependently inhibits calcium mobilization induced by both GROα and IL-8,with IC50 values of 20 and 40nM,respectively.WHCO1 cells treated with SB225002 exhibits a 40% reduction in cell proliferation. Blocking CXCR2 signaling in WHCO1 cells with 400nM SB225002 (SB 225002) significantly decreases cell proliferation by ~40% to 50%.
体内研究	SB225002 (SB 225002) selectively blocks IL-8-induced neutrophil margination in rabbits.CXCR2 is blocked using the selective antagonist SB225002 (2mg/kg) or neutralizing CXCR2 antiserum.The CXCR2 antagonist SB225002 decreases neutrophil counts in ischemic hemispheres of ApoE−/−mice on Western diet and wildtype mice on normal diet.SB225002 significantly attenuates microglial activation and BBB damage,increases myelination,and reduces astrogliosis in the white matter after LPS-sensitized HI.

推荐实验方法(仅供参考)

激酶实验：

CHO-CXCR1 and CHO-CXCR2 membranes are prepared.Assays are performed in 96-well microtiter plates where the reaction mixture contained 1.0μg/mL membrane protein in 20mM Bis-Tris-propane, pH 8.0,with 1.2mM MgSO4,0.1mM EDTA,25mM NaCl,and 0.03% CHAPS and SB 225002 (10mM stock in Me2SO) added at the indicated concentrations,the final Me2SO concentration is <1% under standard binding conditions. Binding is initiated by addition of 0.25nM 125I-IL-8 (2,200 Ci/mmol).After 1-h incubation at room temperature the plate is harvested using a Tomtec 96-well harvester onto a glass fiber filtermat blocked with 1% polyethyleneimine,0.5% BSA and washed three times with 25mM NaCl,10mM Tris•HCl,1mM MgSO4,0.5mMEDTA,0.03% CHAPS,pH 7.4.The filter is dried,sealed in a sample bag containing 10mL of Wallac 205 Betaplate liquid scintillation fluid,and counted with a Wallac 1205 Betaplate liquid scintillation counter.

细胞实验:

Three esophageal squamous cell carcinoma cell lines WHCO1,WHCO5,and WHCO6 originally established from surgical biopsies of primary esophageal squamous cell carcinomas are cultured in DMEM containing 10% FCS at 37℃ in a humidified atmosphere of 5% CO2.MTT assays are carried out using the Cell Proliferation kit.Briefly,1.5×103 cells are plated in 96-well plates in a final volume of 180μL DMEM per well.SB 225002 (400nM) is added to cells and 0.001% DMSO (solvent) is added as a control.After the indicated incubation period,18μL of the MTT labeling reagent (final concentration 0.5mg/mL) is added to each well and incubated for 4 hours in a humidified atmosphere.One hundred eighty microliters of the solubilization solution are added to each well and the plates are left overnight at 37℃.The spectrophotometric absorbance of samples is measured at 595 nm using a microtiter plate reader.

动物实验:

Rats

In this study,10-12 Sprague-Dawley rat pups per dam are used.The pups receive intraperitoneal injections of SB225002 (1 or 3mg/kg,diluted in NS containing 0.33 % Tween 80) or vehicle (NS solution containing 0.33 % Tween 80) 30 min before lipopolysaccharide (LPS) administration and immediately after hypoxic ischemia (HI). The pups are randomly assigned to four groups: control (pups unexposed to LPS or HI, N=14),vehicle (NS injections 30 min before LPS administration and immediately after HI,N=18),and SB-1 (1mg/kg,N=14) and SB-3 (3mg/kg,N=18) (SB225002 injections 30 min before LPS administration and immediately after HI).

保存条件：

2-8℃ 充氮保存

UN码：

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危害声明：

安全说明：

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