中文名称: 荧光素-二-β-D-吡喃半乳糖苷  一键复制产品信息
英文名称: Fluorescein di-β-D-galactopyranoside
CAS No: 17817-20-8
分子式: C32H32O15
分子量: 656.59
F10147 荧光素-二-β-D-吡喃半乳糖苷 ≥98% (普西唐-psaitong)
包装规格:
1mg 2mg 5mg in glass bottle
溶解性:
溶于甲醇(20mg/mL)
产品描述:

基本信息

产品编号:

F10147

产品名称:

Fluorescein di-β-D-galactopyranoside

CAS:

17817-20-8

 

储存条件

粉末

-20℃

四年

 

 

分子式:

C32H32O15

溶于液体

 

 

分子量

656.59

 

 

化学名: 

3',6'-bis[[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy]spiro[2-benzofuran-3,9'-xanthene]-1-one

 

生物活性

产品描述

Fluorescein di(β-D-galactopyranoside) 是 β-半乳糖苷酶的荧光底物 (λex=485nm,λem=535nm)。

靶点

 

体外研究

The fluorescence produced by Fluorescein di(β-D-galactopyranoside) increases in a time- and dose-dependent manner. The level of fluorescence produced by the double-substrate method is much lower than that by the Fluorescein di(β-Dgalactopyranoside) method. Results show that the fluorescein produced by Fluorescein di(β-D-galactopyranoside) in Hs68 cells is proportional to the number of passages

 

推荐实验方法(仅供参考)

Cell Assay

The cells (5×103 cells per well) are cultured in a 96-well plate overnight for attachment, washed, and then fixed in solutions. An aliquot (100μL) of the reaction buffer (i.e., the staining solution without X-Gal) is added into each well. Then, 10μL of 2mM Fluorescein di(β-D-galactopyranoside) is added per well and the plate is incubated in the dark at 37°C for 24 h without CO2 supply. After incubation at 37°C for 24 h, 100μL of the supernatant is transferred to a 96-well plate for fluorescent measurement in triplicates. The fluorescein fluorescence is measured using a fluorometer with an excitation at 485nm and an emission at 535nm

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
搜索质检报告(COA)

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • =
    *
    *
    *选择对应的单位 *空出希望得到的变量,填写另外两个变量

用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):