基本信息
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产品编号： |
E10090 |
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产品名称： |
Ergosterol |
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CAS: |
57-87-4 |
储存条件 |
粉末 |
2-8℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
两年 |
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分子量 |
396.65 |
-20℃ |
一个月 |
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化学名： |
Ergosta-5,7,22-trien-3-ol, (3b,22E)- |
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Solubility (25°C)： |
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体外:
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DMSO |
0.01mg/mL(0.02mM) |
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Ethanol |
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Water |
Insoluble |
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体内(现配现用): |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
2.5211mL |
12.6056mL |
25.2111mL |
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5mM |
0.5042mL |
2.5211mL |
5.0422mL |
生物活性
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产品描述 |
维生素 D2 的生物前体，存在于真菌和一些原生生物如锥虫的细胞膜中。麦角甾醇可用于抗真菌药物如两性霉素 B 及其类似物的功能研究以及各种真菌中麦角甾醇生物合成途径的研究。 |
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靶点 |
Human Endogenous Metabolite |
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体外研究 |
Ergosterol is a sterol isolated from Grifola frondosa, which can be used in the research of mast cell-dependent allergic diseases. Ergosterol (10, 20, 50μM) inhibits the antigen-induced release of β-hexosaminidase and histamine in antigenstimulated RBL-2H3 cells. Ergosterol (20 and 50μM) significantly reduces the mRNA levels of of IL-4 and TNF-α. Ergosterol (50μM) inhibits the antigen-induced aggregation of FcεRI |
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体内研究 |
Ergosterol (25, 50mg/kg, p.o.) significantly mitigates the reduced cardiac performance in rats induced by LPS, increases SOD activity and decreases the formation of MDA, CK-MB, and LDH in LPS-induced sepsis rats |
推荐实验方法(仅供参考)
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Cell Assay |
RBL-2H3 cells are seeded into 24-well plates containing gelatin-coated cover glasses at 0.75 × 105 cells/well and sensitized with anti-DNP IgE. After culture for 24 hours, the cells cultured on cover glasses are pretreated with or without 50μM Ergosterol or 1mM methyl beta cyclodextrin (MβCD) in PIPES buffer for 20 minutes. The cells are then challenged with DNPHSA (50 ng/mL) for 20 minutes. After washing with ice-cold PBS immediately, the cells are fixed with 3.7% formaldehyde in PBS for 20 minutes and blocked with 1% BSA in PBS. The IgE/α-chain of FcεRI complexes on cell surfaces are detected using goat anti-mouse IgE antiserum, and Alexa Fluor 488-conjugated anti-goat IgG. Fluorescence images are acquired using a laser scanning confocal microscope with Zen software. The data are quantified by counting the aggregation number of Fcε RI positive cells and presented as aggregation positive cells/total cells. The cells are counted in six independent micrographs for each sample |
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Animal Administration |
Rats Experimental myocardial injury in rats is performed by LPS injection (15mg/kg). Dexmedetomidine (Dex) is used as a positive control. The experimental animals are randomly divided into five groups (n = 10) as follows: Control group, rats receive 2% gum acacia suspension orally at a dose of 2mL/kg for 5 days, followed by normal saline injected intraperitoneally on day 5; LPS group, rats receive 2% gum acacia suspension at dose of 2mL/kg for 5 days with LPS simultaneously injected intraperitoneally day 5; LPS+ Dex group, rats are treated with 2mg/kg Dex suspension followed by LPS injection on day 5; LPS + Ergosterol (25mg/kg, 50mg/kg) groups, 25 or 50mg/kg Ergosterol are given to rats orally for 5 consecutive days, and LPS is injected on day 5. Twelve hours after LPS treatment, blood samples are collected through the retro-orbital plexus. The serum specimens are centrifugated at 4, 000 × g for 15 min and stored at −80°C until needed. Thereafter, rats are anesthetized and sacrificed. Heart tissues are removed and homogenized in ice-cold phosphate buffered saline (50mM, pH 7.4). Heart tissue homogenates from different groups are centrifuged at 12, 000 × g for 45 min at 4°C and the supernatants retained for further biochemical evaluations |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
