合成辣椒素 - CAS:2444-46-4 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	合成辣椒素一键复制产品信息
英文名称:	Capsaicin
别名:	壬酸香草酰胺 Capsaicin
CAS No:	2444-46-4	分子式:	C₁₇H₂₇NO₃	分子量:	293.41
CAS No:	2444-46-4
分子式:	C₁₇H₂₇NO₃
分子量:	293.41
MDL:	MFCD00017286	EINEC:	219-484-1
EINEC:	219-484-1

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C10198 合成辣椒素 ≥98%(HPLC),分析标准品 (普西唐-psaitong)

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包装规格：

20mg in glass bottle

产品描述：

基本信息

产品编号：	C10198
产品名称：	Capsaicin
CAS:	2444-46-4	储存条件	粉末	2-8℃	四年

分子式：	C17H27NO3		溶于液体	-80℃	两年
分子量	293.41			-20℃	一个月
化学名：	N-(4-Hydroxy-3-methoxybenzyl)nonanamide
Solubility (25°C)：
体外:	DMSO	58mg/mL (197.68mM)
	Ethanol	58mg/mL (197.68mM)
	Water	Insoluble
体内(现配现用):	1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.5mg/mL (8.52mM); Clear solution
	此⽅案可获得 ≥ 2.5mg/mL (8.52mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。
	2.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 2.5mg/mL (8.52mM); Clear solution
	此⽅案可获得 ≥ 2.5mg/mL (8.52mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。
	3.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2.5mg/mL (8.52mM); Clear solution
	此⽅案可获得 ≥ 2.5mg/mL (8.52mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	3.4083mL	17.0416mL	34.0832mL
5mM	0.6817mL	3.4083mL	6.8166mL
10mM	0.3408mL	1.7042mL	3.4083mL
50mM	0.0682mL	0.3408mL	0.6817mL

生物活性

产品描述	Nonivamide 是⼀种激动剂，在静态毒性检测中，4d-EC50 为 5.1mg/L。
靶点	TRPV1
体外研究	Nonivamide, a synthetic derivate of natural capsaicin, has an effective antifouling activity. Capsaicin exhibits 4d-EC50 values of 5.5±0.5mg/L, 23±2mg/L, 6.9±0.2mg/L, and 15.6±0.4mg/L in static toxicity tests conducted using Pseudomonas putida, Lake Erie bacteria, Vibrio natriegens, and Vibrio parahaemolyticus, respectively. A significant growth inhibitory effect (p<0.01) is observed in the group treated with 1mg/L of Nonivamide for 4 d, and the EC50 value (4 d-EC50) is 5.1mg/L[1] . Nonivamide treatment causes calcium release from the ER and altered the transcription of growth arrest- and DNA damageinducible transcript 3 (GADD153), GADD45α, GRP78/BiP, ATF3, CCND1, and CCNG2) in a manner comparable with prototypical ER stress-inducing agents. ER calcium flux is evaluated by pretreating cells with 2.5µM thapsigargin for 5 min followed by addition of 2.5µM Nonivamide. Treatment of TRPV1-overexpressing cells with 2.5µM Nonivamide produces marked increases in cytosolic calcium due to release of calcium from ER stores. Treatment of TRPV1-overexpressing cells with 1µM Nonivamide causes an approximate 50% loss in cell viability after a 24-h period. BEAS-2B cells treated with 100 and 200µM Nonivamide also exhibits a shift in the relative amount of EIF2α-P and an increase in the expression of GADD153 mRNA and protein[2]. Treatment with Nonivamide reduces lipid accumulation to a similar extent as CAP; the effects are not different from the effects after CAP treatment at any of the tested concentrations. Compared to untreated control cells, treatment with Nonivamide decreases lipid accumulation by 5.34±1.03% (P<0.05) at 0.01µM up to 10.4±2.47% (P<0.001) at 1µM

推荐实验方法(仅供参考)

Cell Assay

In the MTT assay, the reduction of yellow tetrazolium salt MTT to a purple formazan by mitochondrial and ER enzymes is used as a measure for cell viability. Cells are seeded in 96‐well plates and treated with 1nm-10µM CAP or Nonivamide with or without addition of 25-100µM BCH or the corresponding ethanol concentration (0.1-0.2% (v/v), solvent control) for 12 days after initiation of differentiation. Cell culture media is exchanged every second day. On Day 12, 100μL of the MTT working reagent (0.83mg/mL MTT diluted in PBS/serum-free media (1:5)), is added to each well, and cells are incubated at 37°C for approximately 15 min. The MTT working solution is removed and the purple formazan formed during incubation is dissolved in 150μL DMSO per well. Absorbance is measured at 550nm with 690nm as reference wavelength using multiwell plate reader. The number of metabolically active cells is calculated relative to untreated control cells or the corresponding solvent control (100%)

保存条件：

2-8℃

UN码：

HazardClass：

危害声明：

安全说明：

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