| 中文名称: | Aldoxorubicin | ||||
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| 英文名称: | Aldoxorubicin | ||||
| 别名: | Aldoxorubicin N-[(E)-[1-[(2S,4S)-4-[(2R,4S,5S,6S)-4-amino-5-hydroxy-6-methyloxan-2-yl]oxy-2,5,12-trihydroxy-7-methoxy-6,11-dioxo-3,4-dihydro-1H-tetracen-2-yl]-2-hydroxyethylidene]amino]-6-(2,5-dioxopyrrol-1-yl)hexanamide | ||||
| CAS No: | 1361644-26-9 | 分子式: | C37H42N4O13 | 分子量: | 750.748390674591 |
| CAS No: | 1361644-26-9 | ||||
| 分子式: | C37H42N4O13 | ||||
| 分子量: | 750.748390674591 | ||||
基本信息
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产品编号： |
A11022 |
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产品名称： |
Aldoxorubicin |
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CAS: |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
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分子量 |
750.75 |
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化学名： |
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Solubility (25°C)： |
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体外:
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DMSO |
55mg/mL (73.26mM; Need ultrasonic) |
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Ethanol |
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Water |
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体内(现配现用): |
1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline， Solubility:≥2.5mg/mL(3.33mM);Clear solution |
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此⽅案可获得≥2.5mg/mL(3.33mM，饱和度未知)的澄清溶液。以1mL⼯作液为例，取100μL25.0mg/mL的澄清DMSO储备液加到400μLPEG300中，混合均匀；向上述体系中加⼊50μLTween-80，混合均匀；然后继续加⼊450μL⽣理盐⽔定容⾄1mL。 |
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2.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility:≥2.5mg/mL(3.33mM);Clear solution |
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此⽅案可获得≥2.5mg/mL(3.33mM，饱和度未知)的澄清溶液。以1mL⼯作液为例，取100μL25.0mg/mL的澄清DMSO储备液加到900μL20%的SBE-β-CD⽣理盐⽔⽔溶液中，混合均匀。 |
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3.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility:≥2.5mg/mL(3.33mM);Clear solution |
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此⽅案可获得≥2.5mg/mL(3.33mM，饱和度未知)的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以1mL⼯作液为例，取100μL25.0mg/mL的澄清DMSO储备液加到900μL⽟⽶油中，混合均匀。 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
1.3320mL |
6.6600mL |
13.3200mL |
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5mM |
0.2664mL |
1.3320mL |
2.6640mL |
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10mM |
0.1332mL |
0.6660mL |
1.3320mL |
生物活性
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产品描述 |
一种多柔比星的白蛋白结合前药，在酸性条件下从白蛋白中释放出来。 |
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靶点 |
Topoisomerase II |
Daunorubicins/Doxorubicins |
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体外研究 |
Aldoxorubicin (INNO-206) (0.27 to 2.16μM) inhibits blood vessel formation and reduces multiple myeloma cell growth in a pH-dependent fashion |
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体内研究 |
Aldoxorubicin (INNO-206) (10.8mg/kg, i.v.) shows significantly smaller tumor volumes and IgG levels on days 28, and is well tolerated with 90% of mice surviving until the termination of the study in the mice bearing the LAGκ-1A tumor . Aldoxorubicin (INNO-206) shows a good safety profile at doses up to 260mg/mL doxorubicin equivalents, and is able to induce tumor regressions in breast cancer, small cell lung cancer and sarcoma in phase I study. Aldoxorubicin (INNO-206) shows superior activity over doxorubicin in a murine renal cell carcinoma model and in breast carcinoma xenograft models |
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推荐实验方法(仅供参考)
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Cell Assay |
Cells are seeded at 1×105 cells/100μL/well in 96-well plates in RPMI-1640 media with FBS for 24 hours before treatment. Cells are cultured in the presence of medium, Aldoxorubicin (INNO-206) or doxorubicin for 48 hours. Next, cell viability is quantified using the CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay. Each well is treated with MTS for 1 to 4 hours, after which absorbance at 490 nm is recorded using a 96-well plate reader. The quantity of formazan product as measured is directly proportional to the number of living cells. Data graphed are means±SEM using 3 replicates per data point |
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Animal Administration |
For the LAGκ-1A experiment, Aldoxorubicin (INNO-206) is administered to SCID mice at 10.8mg/kg (doxorubicin equivalent dose of 8.0mg/kg) once weekly. Mice are treated with conventional doxorubicin at 4.0 and 8.0mg/kg once weekly. For the LAGκ-2 experiment, Aldoxorubicin (INNO-206) is administered once weekly (W) at doses of 2.7 and 5.4mg/kg, or on 3 consecutive days (W-F) weekly at doses of 0.9 and 1.8mg/kg. PS-341 is administered twice weekly (W, F) at a dose of 0.5mg/kg. Doxorubicin is administered to SCID mice at 2, 4, and 8mg/kg, and PLD is administered to SCID mice at 2mg/kg once weekly. Each drug is administered i.v. in a volume of 100μL |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
