中文名称: T-00127_HEV1
英文名称: T-00127_HEV1
CAS No: 900874-91-1
分子式: C22H29N5O3
分子量: 411.5
T11485 T-00127_HEV1 ≥98% (psaitong)
包装规格:
1mg 5mg in glass bottle
溶解性:
溶于DMSO(50mg/mL超声)
产品描述:

基本信息

产品编号:

T11485

产品名称:

T-00127_HEV1

CAS:

900874-91-1

 

储存条件

粉末

-20℃

四年

分子式:

C22H29N5O3

溶于液体

-80℃

6个月

分子量:

411.50

-20℃

1个月

化学名: 

3-(3,4-Dimethoxyphenyl)-2,5-dimethyl-N-(2-morpholin-4-ylethyl)pyrazolo[1,5-a]pyrimidin-7-amine

Solubility (25°C):

 

体外:

 

DMSO

 

Ethanol

 

Water

 

体内(现配现用):

 

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

2.4301mL

12.1507mL

24.3013mL

5mM

0.4860mL

2.4301mL

4.8603mL

10mM

0.2430mL

1.2151mL

2.4301mL

 

生物活性

产品描述

一种phosphatidylinositol 4-kinase III beta (PI4KB)抑制剂,其IC50 值为60nM。

靶点

PI4KB 60nM (IC50)

 

体外研究

T-00127_HEV1 shows more potent anti-poliovirus (PV) activity (EC50 of 0.77μM) than other candidate compounds (EC50 of 1.7 to 4.7μM).GW5074 and T-00127_HEV1 almost completely inhibit PI4KB kinase activity at 10μM (3% and 5% of residual activity,respectively),in contrast to AN-12-H5 (108% of activity [no inhibition]).

 

推荐实验方法(仅供参考)

激酶实验:

The inhibitory effect of T-00127_HEV1 at a concentration of 10μM on in vitro cellular protein kinase activities is assessed by kinase profiling with an ATP concentration near the Km for each kinase.Inhibitory effects of GW5074,AN-12-H5,and T00127_HEV1 at a concentration of 10μM on in vitro PI kinase activities are assessed by the SelectScreen kinase profiling service with an ATP concentration of 10μM.For T-00127_HEV1,the 50% inhibitory concentration (IC50) for in vitro PI4KB activity is also measured with an ATP concentration of 10μM.

 

细胞实验:

 

RD cells (1.0×104 cells per well in 100μL medium) in a 96-well plate are infected with PV1,EV71,or CVB3 at multiplicities of infection (MOI) of 10,1.0,and 0.1 at 37℃ for 1h in the absence of T-00127_HEV1. The cells are washed three times with 10% FCS-DMEM, followed by the addition of 100μL of 10% FCS-DMEM containing 10 or 0μM T-00127_HEV1.Cells are collected at 16h p.i.,and then viral RNA is extracted from the cells using a viral RNA purification kit.The number of copies of the viral genome is quantified using a real-time PCR system.

 

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
搜索质检报告(COA)
参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • =
    *
    *
    *选择对应的单位 *空出希望得到的变量,填写另外两个变量

用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):