中文名称: PI4KIIIbeta-IN-10
英文名称: PI4KIIIbeta-IN-10
CAS No: 1881233-39-1
分子式: C22H25N3O5S2
分子量: 475.58
P10567 PI4KIIIbeta-IN-10 ≥98% (psaitong)
包装规格:
1mg 5mg 10mg 25mg in glass bottle
溶解性:
溶于DMSO(≥ 30 mg/mL)
产品描述:

基本信息

产品编号:

P10567

产品名称:

PI4KIIIbeta-IN-10

CAS:

1881233-39-1

 

储存条件

粉末

-20℃

四年

 

 

分子式:

C22H25N3O5S2

溶于液体

-80℃

6个月

分子量:

475.58

-20℃

1个月

化学名: 

PI4KIIIbeta-IN-10;N-(5-(3-(N-(4-hydroxyphenyl)sulfamoyl)-4-methoxyphenyl)-4-methylthiazol-2-yl)pivalamide

Solubility (25°C):

 

体外:

 

DMSO

 

Ethanol

 

Water

 

体内(现配现用):

 

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

2.1027mL

10.5135mL

21.0270mL

5mM

0.4205mL

2.1027mL

4.2054mL

10mM

0.2103mL

1.0513mL

2.1027mL

 

生物活性

产品描述

一种有效的PI4KIIIβ抑制剂

靶点

PI4K

体外研究

PI4KIIIbeta-IN-10 (Compound 10) is a potent PI4KIIIβ inhibitor with very minor off-target inhibition of PI4KIIIβ related lipid kinases.PI4KIIIbeta-IN-10 shows weak inhibition of PI3KC2γ(IC50 ~1µM),PI3Kα(~10 µM),and PI4KIIIα(~3 µM),and <20% inhibition at concentrations up to 20µM for PI4K2α,PI4K2β,and PI3Kβ.

 

推荐实验方法(仅供参考)

激酶实验:

Lipid kinase assays are preformed using recombinant enzyme,phosphoinositides and γ32P-ATP in a membrane capture assay.Each inhibitor (e.g.,PI4KIIIbeta-IN-10) is diluted into 10% DMSO and kinase assay buffer.Upon completion of the reaction,4µL is spotted onto 0.2µm nitrocellulose.The membrane is dried for 5 minutes under a heat lamp followed by 1×30 second wash and 6×5 min washes in 1M NaCl/1% Phosphoric Acid.The membrane is dried for 20 minutes under a heat lamp followed by overnight exposure to a phosphor screen and phosphorimaging followed on a Typhoon 9500.Intensities are quantified using SPOT. Specifications for each enzyme follow.L-α-Phosphatidylinositol and DOPS:DOPC lipids are sonicated in water to generate 1mg/mL PI:DOPS:DOPC.Reaction is set-up as follows 1) kinase assay buffer,PI:DOPS:DOPC,BSA and PI4KIIIβ,are combined in a total volume of 10µL (2.5x solution);2) 5µL of inhibitor solution is added (5x solution) and incubated with enzyme mixture for 15 minutes;3) 10µL cold ATP and γ32P-ATP are added (2.5x solution) to initiate the reaction which ran for 30 minutes.Final conditions are as follows:20mM Bis-Tris Propane pH 7.5,10mM MgCl2,0.075mM Triton X-100,0.5mM EGTA,1mM DTT,100µM PI,500ng/µL BSA,2.5nM PI4KIIIβ,2% DMSO,10µM ATP and 1 uCi γ32P-ATP.

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

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    *
    *选择对应的单位 *空出希望得到的变量,填写另外两个变量

用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):