Spautin-1 - CAS:1262888-28-7 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	Spautin-1 促销
英文名称:	Spautin-1
别名:	4-((4-氟苄基)氨基)-6-氟喹唑啉;Spautin-1 抑制剂 6-Fluoro-N-[(4-Fluorophenyl)methyl]-4-quinazolinamine
CAS No:	1262888-28-7	分子式:	C₁₅H₁₁F₂N₃	分子量:	271.26
CAS No:	1262888-28-7
分子式:	C₁₅H₁₁F₂N₃
分子量:	271.26

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S10684 Spautin-1 ≥98% (psaitong)

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包装规格：

10mg 50mg 100mg in glass bottle

溶解性：

溶于DMSO（50 mg/mL 超声）

产品描述：

基本信息

产品编号：S10684
产品名称：Spautin-1
CAS:	1262888-28-7	储存条件	粉末	-20℃	四年

分子式：	C15H11F2N3		溶于液体	-80℃	两年
分子量	271.26			-20℃	一个月
化学名：	6-Fluoro-N-[(4-Fluorophenyl)methyl]-4-quinazolinamine
Solubility (25°C)	体外	DMSO	54mg/mL (199.07mM)
		Ethanol	7mg/mL (25.8mM)
		Water	Insoluble
	体内	现配现用
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	3.6865mL	18.4325mL	36.8650mL
5mM	0.7373mL	3.6865mL	7.3730mL
10mM	0.3687mL	1.8433mL	3.6865mL
50mM	0.0737mL	0.3687mL	0.7373mL

生物活性

产品描述	一种特异性和有效的自噬抑制剂，可抑制泛素特异性肽酶USP10和 USP13，IC50s 约为0.6-0.7μM。
靶点/IC₅₀	USP10 (Cell-free assay) 0.5779μM	USP13 (Cell-free assay) 0.6916μM
体外研究	Spautin-1 enhances imatinib mesylate (IM)-induced CmL cell apoptosis by reducing the expression of the anti-apoptotic proteins Mcl-1 and Bcl-2. The pro-apoptotic activity of spautin-1 is associated with activation of GSK3β, an important downstream effector of PI3K/AKT. Spautin-1 enhances IM-induced cytotoxicity in CmL cell line K562, decreasing the IC50 from 1 to 0.5 μM. The mechanism of spautin-1 acting on acute pancreatitis is associated with impaired autophagy inhibition.
体内研究	Spautin-1 ameliorates the pathogenesis of acute pancreatitis induced by cerulein or L-arginine. Spautin-1 pretreatment significantly diminishes the elevation of serum amylase and lipase levels, which are indicative of trypsin activity. Increasing levels of serum TNFα caused by cerulein are inhibited in the presence of spautin-1. Spautin-1 treatment can ameliorate the inflammation damage induced by cerulein, such as edema, degeneration, coagulative necrosis and infiltration of inflammatory cells.

推荐实验方法(仅供参考)

细胞实验：
Cell Assay	Spautin-1 is dissolved in DMSO. Cell proliferation is evaluated using CCK-8 kit. K562 cells (1x105/mL) are seeded into 96-well plates in triplicate and then treated with 125 to 4,000nM IM alone or in combi¬nation with spautin-1 (10μM). After 48 h of incubation, 10μL of CCK-8 reagent is added to each well. Four hours later, the absorbance is read at 450nm using a microplate reader.

动物实验:

Animal Administration

Mice: In this study, mice models with acute pancreatitis, including cerulein- and L-arginine-induced models, are constructed. For the cerulein-induced model, four intraperitoneal injections of cerulein (50μg/kg body weight) are given consecutively at hourly intervals; The L-arginine-induced model received hourly intraperitoneal injections of 1.4 g/kg(optimal dosage for this study) L-arginine three times.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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