| 中文名称: | NL-1 | ||||
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| 英文名称: | NL-1 | ||||
| 别名: | NL-1;QDYYSPGKYXWGSL-UHFFFAOYSA-N;5-(3,5-Di-tert-butyl-4-hydroxybenzyl)thiazolidine-2,4-dione;5-[3,5-bis(1,1-dimethylethyl)-4-hydroxyphenyl]methyl-2,4-thiazolidinedione | ||||
| CAS No: | 188532-26-5 | 分子式: | C18H25NO3S | 分子量: | 335.46 |
| CAS No: | 188532-26-5 | ||||
| 分子式: | C18H25NO3S | ||||
| 分子量: | 335.46 | ||||
基本信息
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产品编号：N10646 |
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产品名称：NL-1 |
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CAS: |
188532-26-5 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
335.46 |
-20℃ |
一个月 |
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化学名： |
5-[3,5-bis(1,1-dimethylethyl)-4-hydroxyphenyl]methyl-2,4-thiazolidinedione |
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Solubility (25°C) |
体外 |
DMSO |
200mg/mL (596.20mM; Need ultrasonic) |
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Ethanol |
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Water |
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体内 |
现配现用 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
2.9810mL |
14.9049mL |
29.8098mL |
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5mM |
0.5962mL |
2.9810mL |
5.9620mL |
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10mM |
0.2981mL |
1.4905mL |
2.9810mL |
生物活性
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产品描述 |
一种具有抗白血病作用的 mitoNEET 抑制剂。NL-1 抑制 REH 和 REH/Ara-C 细胞生长，IC50 分别为 47.35μM 和 56.26μM。NL-1 介导的白血病细胞死亡需要激活自噬途径。 |
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靶点/IC50 |
MitoNEET |
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体外研究 |
NL-1 (10-100μM; 72 hours; REH, REH/Ara-C and ALL cell lines) treatment reduces the number of viable cells in REH, REH/AraC and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines, in a concentration-dependent manner. NL-1 inhibits SUPB15, NALM6 with IC50s of 29.48 µM, 94.26 µM, respectively. TOM1, BV173, NALM1 and JM1 all have similar IC50 values of around 60 µM for NL-1. NL-1 (60μM; 6 hours; REH, REH/Ara-C cell lines) treatment mediates autophagy, and inhibition of autophagy partially decreased NL-1-induced tumor cell death. NL-1 pretreatment inhibits the chemotactic ability of both REH and REH/Ara-C cells to migrate towards multiple chemoattractants. The cells treated with NL1 shows a dose-dependent decrease in chemotaxis both in the REH and the REH/AraC cells. Cell Proliferation Assay |
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Cell Line: |
REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines |
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Concentration: |
10μM, 20μM, 30μM, 40μM, 50μM, 60μM, 70μM, 80μM, 100μM |
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Incubation Time: |
72 hours |
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Result: |
Reduced the number of viable cells in REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines, in a concentration-dependent manner. |
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Cell Autophagy Assay |
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Cell Line: |
REH, REH/Ara-C cell lines |
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Concentration: |
60μM |
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Incubation Time: |
6 hours |
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Result: |
Induced cell autophagy. |
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体内研究 |
NL-1 (10mg/kg; intraperitoneal injection; daily; for 5 days; female NSG mice) treatment shows antileukemic activity in an in vivo mouse ALL model. |
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Animal Model: |
10 female NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice (6-8 month old) injected with TOM-1 ALL cells |
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Dosage: |
10mg/kg |
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Administration: |
Intraperitoneal injection; daily; for 5 days |
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Result: |
Showed antileukemic activity in an in vivo mouse ALL model. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
