中文名称: | Nesbuvir | ||||
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英文名称: | Nesbuvir | ||||
别名: | Nesbuvir 抑制剂;5-环丙基-2-(4-氟苯基)-6-[(2-羟基乙基)(甲基磺酰基)氨基]-N-甲基-3-苯并呋喃羧酰胺;5-环丙基-2-(4-氟苯基)-6-[(2-羟基乙基)(甲基磺酰基)氨基]-N-甲基-3-苯并呋喃甲酰胺 HCV-796 | ||||
CAS No: | 691852-58-1 | 分子式: | C22H23FN2O5S | 分子量: | 446.49 |
CAS No: | 691852-58-1 | ||||
分子式: | C22H23FN2O5S | ||||
分子量: | 446.49 |
基本信息
产品编号:N10415 |
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产品名称:Nesbuvir |
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CAS: |
691852-58-1 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
446.49 |
-20℃ |
一个月 |
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化学名: |
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Solubility (25°C) |
体外 |
DMSO |
≥50mg/mL (111.98mM) |
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Ethanol |
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Water |
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体内 |
现配现用 |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
2.2397mL |
11.1985mL |
22.3969mL |
5mM |
0.4479mL |
2.2397mL |
4.4794mL |
10mM |
0.2240mL |
1.1198mL |
2.2397mL |
生物活性
产品描述 |
一种非核苷抑制剂,抑制丙型肝炎病毒非结构蛋白聚合酶。 |
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靶点/IC50 |
NS3V170A EC50: 9nM |
NS3V170A 13nM |
NS3K583T 15nM |
NS5BI424V 13nM |
体外研究 |
Replicon cells are treated with 1 mg/mL G418 and combinations of the two compounds. Nesbuvir (HCV-796) is added to 40 or 80nM (approximately 10 and 20 times the EC50 in a 3-day replicon inhibition assay, respectively) and Boceprevir is added to 400 or 800nM (approximately 2 and 4 times the EC50, respectively). The EC50s for Nesbuvir and Boceprevir for the parental replicon in the transient expression assay are comparable to those obtained in the 3-day inhibition assay with the stable replicon cells; the EC50 for Nesbuvir in the transient expression assay is 14nM, whereas it is 5nM for the stable replicon; and the EC50 for Boceprevir in the transient expression assay is 608nM, whereas it is 201nM for the stable replicon. |
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体内研究 |
Among a huge variety of yet characterized nucleoside and non-nucleoside inhibitors (NNI), the benzofurane derivative NNI Nesbuvir (HCV-796) is demonstrated to yield significant antiviral effects in mice with chimeric human livers and in patients infected with HCV. HCV-796 binds to a hydrophobic binding pocket at the “palm” domain of NS5B; however, its mode of inhibition remains to be defined. |
推荐实验方法(仅供参考)
细胞实验: |
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Cell Assay |
Huh7-BB7 cells are seeded at a density of 20,000 cells per 100 mm dish in DMEM supplemented with 2% FBS, 1 mg/mL G418,and various concentrations of Nesbuvir and/or Boceprevir with DMSO at a final concentration of 0.5% (vol/vol). The medium is removed and is replaced with fresh medium with the appropriate compound concentrations every 3 or 4 days. After 7 days, the cells are split 1 to 10, placed into fresh 100 mm dishes, and incubated with medium with the appropriate compound concentrations. After 20 days, the medium is removed and the cells are fixed with 7% (wt/vol) formaldehyde and stained with 1% (wt/vol) crystal violet in 50% (vol/vol) ethanol. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )