中文名称: | Go 6983 | ||||
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英文名称: | Go 6983 | ||||
别名: | 3-[1-[3-(二甲基氨基)丙基]-5-甲氧基-1H-吲哚-3-基]-4-(1H-吲哚-3-基)-1H-吡咯-2,5-二酮;邻苯二甲酸二异辛酯(DIOP);十八水合硼钨酸镉;Go 6983 国华试剂 Gö 6983; Goe 6983 | ||||
CAS No: | 133053-19-7 | 分子式: | C26H26N4O3 | 分子量: | 442.51 |
CAS No: | 133053-19-7 | ||||
分子式: | C26H26N4O3 | ||||
分子量: | 442.51 |
基本信息
产品编号:G10433 |
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产品名称:Go 6983 |
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CAS: |
133053-19-7 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
六个月 |
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分子量: |
442.51 |
-20℃ |
一个月 |
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化学名: |
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Solubility (25°C) |
体外 |
DMSO |
88mg/mL (198.86mM) |
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Ethanol |
Insoluble |
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Water |
Insoluble |
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体内(现配现用) |
30% PEG400+0.5% Tween80+5% propylene glycol |
30mg/mL |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
2.2598mL |
11.2992mL |
22.5984mL |
5mM |
0.4520mL |
2.2598mL |
4.5197mL |
10mM |
0.2260mL |
1.1299mL |
2.2598mL |
50mM |
0.0452mL |
0.2260mL |
0.4520mL |
生物活性
产品描述 |
一种有效的 PKC 抑制剂,能够作用于 PKCα,PKCβ,PKCγ,PKCδ 和 PKCζ,IC50 值分别为 7nM,7nM,6nM,10nM 和 60nM。 |
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靶点/IC50 |
PKCγ |
PKCα |
PKCβ |
PKCδ |
PKCζ |
6nM |
7nM |
7nM |
10nM |
60nM |
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体外研究 |
Go 6983 inhibits PKCμwith IC50 of 20μM,and the pther PKC isoenzymes can be suppressed by Go 6983 with IC50 values from 7 to 60nM.Go 6983 (100nM) significantly reduces PMN adherence to the endothelium and infiltration into the myocardium compared with I/R + PMN hearts,and significantly inhibits superoxide release from PMNs by 90 +/- 2% in rat heart. Go 6983 (200nM) has a reduced cardioprotective effect compared with the cardioprotective Go 6983 concentrations (50 and 100nM) despite inhibiting PMN superoxide release by 99%. |
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体内研究 |
在小鼠肺B16BL6肿瘤模型中,Go6983 (22.0μg/mouse,i.v.)强烈抑制51.2 %的肿瘤转移。 |
推荐实验方法(仅供参考)
激酶实验: |
Phosphorylation reactions are carried out in a total volume of 100μL,containing buffer C (50mM Tris-HCl,pH 7.5,10mM βmercaptoethanol),4mM MgCl2,10μg PS, 100 nM TPA,5μL of a Sf158 cell extract as a source of recombinant PKCμor of Sf9 cell extracts as a source of other recombinant PKC isoenzymes,10μg of syntide 2 as substrate,and 35μM ATP containing 1μCi [γ-32P]ATP.In some experiments,PS and TPA are omitted or various inhibitors at concentrations indicated in the text are added. After incubation for 10 min at 30℃, the reaction is terminated by transferring 50μL of the assay mixture onto a 20mm square piece of phosphocellulose paper,which is washed 3 times in deionized water and twice in acetone.The radioactivity on each paper is determined by liquid scintillation counting. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )