中文名称: | Erastin 热销 | ||||
---|---|---|---|---|---|
英文名称: | Erastin | ||||
别名: | 2-(1-(4-(2-(4-氯苯氧基)乙酰基)哌嗪-1-基)乙基)-3-(2-乙氧基苯基)喹唑啉-4(3H)-酮 2-(1-(4-(2-(4-Chlorophenoxy)acetyl)piperazin-1-yl)ethyl)-3-(2-ethoxyphenyl)quinazolin-4(3H)-one | ||||
CAS No: | 571203-78-6 | 分子式: | C30H31ClN4O4 | 分子量: | 547.04 |
CAS No: | 571203-78-6 | ||||
分子式: | C30H31ClN4O4 | ||||
分子量: | 547.04 |
基本信息
产品编号:E10253 |
|||||
产品名称:Erastin |
|||||
CAS: |
571203-78-6 |
储存条件 |
粉末 |
-20℃ |
四年 |
分子式: |
溶于液体 |
4℃ |
两年 |
||
分子量 |
547.04 |
|
|
||
化学名: |
|
||||
Solubility (25°C) |
体外 |
DMSO |
15mg/mL warmed with 50ºC water bath (27.42mM) |
||
Ethanol |
Insoluble |
||||
Water |
Insoluble |
||||
体内(现配现用) |
5%DMSO+40%PEG 300+5%Tween80+ddH2O |
1.75mg/mL |
|||
<1mg/ml表示微溶或不溶。 |
|||||
普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
|||||
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
1.8280mL |
9.1401mL |
18.2802mL |
5mM |
0.3656mL |
1.8280mL |
3.6560mL |
10mM |
0.1828mL |
0.9140mL |
1.8280mL |
生物活性
产品描述 |
一种铁诱导的细胞死亡(ferroptosis) 诱导剂。Erastin 结合且抑制电压依赖性阴离子通道。 |
靶点/IC50 |
Ferroptosis |
体外研究 |
Erasrastin triggers oxidative, iron-dependent cell death. Treatment of NRAS-mutant HT-1080 fibrosarcoma cells with the RSL molecule erastin (10µM) results in a time-dependent increase in cytosolic and lipid ROS beginning at 2 hours. Cell death triggered by erastin is significantly inhibited by antioxidants (e.g.,α-tocopherol,butylated hydroxytoluene, and β-carotene) and iron chelators, suggesting that ROS- and iron-dependent signaling is required for erastin-induced ferroptosis. Erastin can directly bind to VDAC2/3 in BJeLR cells. Knockdown of VDAC2 and VDAC3,but not VDAC1, leads to erastin resistance.Erastin has the ability to reduce glutathione level by directly inhibiting cystine/glutamate antiporter system Xc− activity, with activation of the ER stress response. Erastin potently inhibits HT-29 cell survival. Erastin shows a dose-dependent effect,and 30μM of erastin displays the most dramatic effect. |
体内研究 |
Intraperitoneal injection of erastin at well-tolerated doses dramatically inhibits HT-29 xenograft growth in severe combined immunodeficient mice. |
推荐实验方法(仅供参考)
细胞实验: |
|
To test erastin’s activity on colorectal cancer cell survival, HT-29 cells are treated with increasing concentrations of erastin (0.1–30μM). MTT assay was performed. |
动物实验: |
|
Mice: Mice are treated daily with 10 or 30mg/kg body weight of erastin (intraperitoneal injection,for 4 weeks) or vehicle control (Saline). Tumor volumes are calculated. Mice body weights are also recorded every week. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )