中文名称: | DC-05 | ||||
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英文名称: | DC-05 | ||||
别名: | DC_05;DC-05 | ||||
CAS No: | 890643-16-0 | 分子式: | C25H25N3O | 分子量: | 383.49 |
CAS No: | 890643-16-0 | ||||
分子式: | C25H25N3O | ||||
分子量: | 383.49 |
基本信息
产品编号:D10837 |
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产品名称:DC-05 |
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CAS: |
890643-16-0 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
六个月 |
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分子量: |
383.49 |
-20℃ |
一个月 |
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化学名: |
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Solubility (25°C) |
体外 |
DMSO |
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Ethanol |
'mg/mL |
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Water |
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体内(现配现用) |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
2.6076mL |
13.0381mL |
26.0763mL |
5mM |
0.5215mL |
2.6076mL |
5.2153mL |
10mM |
0.2608mL |
1.3038mL |
2.6076mL |
生物活性
产品描述 |
一种 DNMT1 抑制剂,IC50 和 Kd 值分别为 10.3μM 和 1.09μM。 |
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靶点/IC50 |
DNMT1 |
DNMT1 |
PRMT1 37.1μM (IC50) |
体外研究 |
DC-05 is a DNA methyltransferase 1 (DNMT1) inhibitor,with an IC50 and a Kd of 10.3μM and 1.09μM,respectively. DC-05 shows less potent activities against DNMT3A,DNMT3B,G9a,SUV39H1,MLL1,SET7/9,and PRMT1 (IC50:>200,>200,>150,>150,>150,>150, 37.1μM). DC-05 (1.25,2.5,5,and 10μM) potently inhibits the proliferation of HCT116 (human colon cancer) and Capan-1 (human pancreatic adenocarcinoma cells) after treatment for 24,48,and 72h. |
推荐实验方法(仅供参考)
激酶实验: |
To measure the effects of DC-05 on mouse DNMT1 activity,200nM purified DNMT1 is incubated with 200μM of DC-05 and Sadenosylmethionine (AdoMet) in the DNMT assay buffer in the assay plate at 37℃ for 2h.Next,every sample is incubated with the capture and detection antibody,followed by incubation with developer solution for 10 min at room temperature.The absorbance is measured at 450nm using a microplate reader. S-Adenosylhomocysteine (AdoHcy) is used as a positive control. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )