| 中文名称: | CA-5f | ||||
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| 英文名称: | CA-5f | ||||
| 别名: | CA-5f;BCP30170;(E)-3-((1H-Indol-3-yl)methylene)-5-((E)-3,4-dimethoxybenzylidene)-1-methylpiperidin-4-one;CA 5f;CA5f;(3E,5E)-3-(3,4-dimethoxybenzylidene)-5-[(1H-indol-3-yl)methylene]-1-methylpiperidin-4-one;(3E,5E)-3-[(3,4-Dimethoxyphenyl)methylidene]-5-(1H-indol-3-ylmethylidene)-1-methylpiperidin-4-one | ||||
| CAS No: | 1370032-19-1 | 分子式: | C24H24N2O3 | 分子量: | 388.46 |
| CAS No: | 1370032-19-1 | ||||
| 分子式: | C24H24N2O3 | ||||
| 分子量: | 388.46 | ||||
基本信息
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产品编号：C10821 |
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产品名称：CA-5f |
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CAS: |
1370032-19-1 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
两年 |
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分子量 |
388.46 |
-20℃ |
一个月 |
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化学名： |
(E)-3-((1H-Indol-3-yl)methylene)-5-((E)-3,4-dimethoxybenzylidene)-1-methylpiperidin-4-one |
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Solubility (25°C) |
体外 |
DMSO |
78mg/mL (200.79mM) |
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Ethanol |
Insoluble |
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Water |
Insoluble |
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体内 |
现配现用 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
2.5743mL |
12.8713mL |
25.7427mL |
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5mM |
0.5149mL |
2.5743mL |
5.1485mL |
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10mM |
0.2574mL |
1.2871mL |
2.5743mL |
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50mM |
0.0515mL |
0.2574mL |
0.5149mL |
生物活性
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产品描述 |
一种有效的晚期巨自噬/自噬 (macroautophagy/autophagy) 抑制剂，通过抑制自噬体-溶酶体融合起作用。 |
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靶点/IC50 |
Macroautophagy/autophagy |
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体外研究 |
CA-5f (0-40μM, 6 hour) concentration- and time-dependently elevates the level of LC3B-II (a marker to monitor autophagy)and SQSTM1 protein both in A549 cells and HUVECs. CA-5f (20μM, 6 hours) inhibits the degradation of autophagosomes when treated alone or in combination Bafilomycin A1(100nM) or Chloroquine (30μM) in A549 cells and HUVECs. CA-5f (20μM) neither impairs the hydrolytic function nor the quantity of lysosomes. CA-5f (20μM, 96 hours) inhibits the growth of A549 cells, and less cytotoxic to normal HUVECs. Cell Viability Assay |
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Cell Line: |
A549, HUVECs |
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Concentration: |
20μM |
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Incubation Time: |
96 hours |
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Result: |
Exhibited more cytotoxicity against A549 cells compared with normal HUVECs. |
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Western Blot Analysis |
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Cell Line: |
A549 cells and HUVECs |
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Concentration: |
0-40μM |
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Incubation Time: |
6 hours |
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Result: |
Elevated LC3B-II (a marker to monitor autophagy) and SQSTM1 protein levels in a concentration- and time-dependent manner. |
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体内研究 |
CA-5f (40 mg/kg, i.p., every 2 days for up to 30 days) is well tolerated, and potently inhibits the growth of tumor in nude mice bearing A549 lung cancer cells. CA-5f (40 mg/kg, i.p.) suppresses autophagic flux and induces apoptosis in nude mice bearing A549 lung cancer cells. |
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Animal Model: |
Nude mice bearing A549 lung cancer cells |
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Dosage: |
40 mg/kg |
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Administration: |
Injected via caudal vein, every 2 days for up to 30 days |
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Result: |
Significantly suppressed tumor volume and weight in mice, increased the number of apoptotic cells in mice. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
