| 中文名称: | 鸦胆子苦醇 | ||||
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| 英文名称: | Brusatol | ||||
| 别名: | 鸦胆子苦醇 Brusatol | ||||
| CAS No: | 14907-98-3 | 分子式: | C26H32O11 | 分子量: | 520.53 |
| CAS No: | 14907-98-3 | ||||
| 分子式: | C26H32O11 | ||||
| 分子量: | 520.53 | ||||
| MDL: | MFCD01746220 | ||||
基本信息
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产品编号： |
B10088 |
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产品名称： |
Brusatol |
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CAS: |
14907-98-3 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量： |
520.53 |
-20℃ |
1个月 |
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化学名： |
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Solubility (25°C)： |
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体外:
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DMSO |
100mg/mL (192.11mM) |
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Ethanol |
Insoluble |
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Water |
Insoluble |
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体内(现配现用): |
请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照In Vitro⽅式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐是指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶 1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility:≥2.5mg/mL(4.80mM);Clear solution 2. 请依序添加每种溶剂：10% DMSO→90%(20% SBE-β-CD in saline) Solubility:≥2.5mg/mL(4.80mM);Clear solution 3.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility:≥2.5mg/mL(4.80mM);Clear solution |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
1.9211mL |
9.6056mL |
19.2112mL |
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5mM |
0.3842mL |
1.9211mL |
3.8422mL |
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10mM |
0.1921mL |
0.9606mL |
1.9211mL |
生物活性
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产品描述 |
一种来源于植物的天然类西林类药物，通过抑制细胞蛋白的从头合成，在肿瘤培养中表现出广泛的细胞毒性。 |
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靶点 |
Nrf2 |
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体外研究 |
Brusatol (0.05,0.15,0.45,1.35,4.05 and 12.15μg/mL) reduces the viability of CT-26 cells in a dose-dependent manner with IC50 value of 0.27±0.01μg/mL.When Brusatol is combined with Cisplatin (CDDP) at a constant concentration ratio of 1:1,cell growth inhibition is markedly enhanced compared with single-agent treatment;the IC50 value of Brusatol and CDDP cotreatment is 0.19±0.02μg/mL.Brusatol provokes a rapid and transient depletion of Nrf2 protein,through a posttranscriptional mechanism,in mouse Hepa1c1c7 hepatoma cells.Brusatol sensitizes mammalian cells to chemical toxicity-implications for therapeutic targeting of Nrf2. Cell Viability Assay |
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Cell Line: |
The murine CT-26 CRC cell line |
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Concentration: |
0.05,0.15,0.45,1.35,4.05 and 12.15μg/mL |
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Incubation Time: |
48 hours |
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Result: |
The viability of CT-26 cells was reduced in a dose-dependent manner,with IC50 value of 0.27±0.01μg/mL. |
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Western Blot Analysis |
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Cell Line: |
Mouse Hepa-1c1c7 hepatoma cells |
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Concentration: |
1,3,10,30,100,300,and 1000nM |
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Incubation Time: |
2 hours |
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Result: |
Provoked the depletion of Nrf2,in a concentration-dependent manner within 2h of exposure to cells. |
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体内研究 |
"Brusatol is able to reach the tumor tissue and inhibit the Nrf2 pathway.Nude mice are injected with A549 cells to induce tumor growth,followed by a single i.p.injection of 2mg/kg Brusatol.Nrf2 protein levels are significantly decreased at 24h or 48h postinjection.Cisplatin (2mg/kg) or Brusatol (2mg/kg) alone does not inhibit tumor growth significantly,whereas in the combination group,tumor size is significantly reduced." |
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Animal Model: |
Athymic nude mice 4-6 wk old bearing A549 xenografts |
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Dosage: |
2mg/kg |
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Administration: |
Treated i.p.;Cisplatin (2mg/kg),Brusatol (2mg/kg),or in combination every other day for a total of five times |
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Result: |
Nrf2 protein levels were significantly decreased at 24h or 48h postinjection.Cisplatin or Brusatol alone did not inhibit tumor growth significantly, whereas in the combination group, tumor size was significantly reduced. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
