中文名称: | CDDO-EA | ||||
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英文名称: | CDDO-EA | ||||
别名: | CDDO ethyl amide; TP319; RTA 405;(4aS,6aR,6bS,8aR,12aS,14aR,14bS)-11-cyano-N-ethyl-2,2,6a,6b,9,9,12a-heptamethyl-10,14-dioxo-1,3,4,5,6,7,8,8a,14a,14b-decahydropicene-4a-carboxamide | ||||
CAS No: | 932730-51-3 | 分子式: | C33H46N2O3 | 分子量: | 518.73 |
CAS No: | 932730-51-3 | ||||
分子式: | C33H46N2O3 | ||||
分子量: | 518.73 |
基本信息
产品编号: |
C11551 |
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产品名称: |
CDDO-EA |
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CAS: |
932730-51-3 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
6个月 |
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分子量: |
518.73 |
-20℃ |
1个月 |
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化学名: |
RTA 405;(4aS,6aR,6bS,8aR,12aS,14aR,14bS)-11-cyano-N-ethyl-2,2,6a,6b,9,9,12a-heptamethyl-10,14-dioxo-1,3,4,5,6,7,8,8a,14a,14b-decahydropicene-4a-carboxamide |
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Solubility (25°C): |
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体外:
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DMSO |
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Ethanol |
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Water |
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体内(现配现用): |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
1.9278mL |
9.6389mL |
19.2779mL |
5mM |
0.3856mL |
1.9278mL |
3.8556mL |
10mM |
0.1928mL |
0.9639mL |
1.9278mL |
生物活性
产品描述 |
一种NF-E2相关因子2/抗氧化反应元件(Nrf2/ARE)激活剂。 |
靶点 |
Nrf2/ARE |
体外研究 |
CDDO-EA potently activates Nrf2/ARE in a cell culture model of ALS and in the G93A SOD1 mouse model of ALS.CDDO-EA is a potent inducer of apoptosis in A549 lung cancer cells,as shown both by PARP cleavage and Annexin staining.CDDO-EA is more potent than CDDO itself as inducers of heme oxygenase-1 (HO-1).In RAW264.7 macrophage-like cells,CDDO-EA is 7- fold more potent than CDDO as suppressors of the ability of IFN-γ to induce iNOS. |
体内研究 |
The survival analysis shows that G93A mice treated with CDDO-EA,compared to G93A littermate controls, lives significantly longer.CDDO-EA treatment increases the life-span by 20.6 days from 124.05±3.7 days to 144.72±8.1 days (16.6%) (p<0.001).In CDDO-EA-treated G93A mice,the age of death is 141.4±5.2 days and the duration from the age of onset to the age of death is 57.6±7.6 days,which means that the age of death from onset is prolonged by 17.5 days (43%). |
推荐实验方法(仅供参考)
细胞实验: |
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Wild-type and Nrf2−/−mouse embryonic fibroblasts are pre-treated with CDDO-EA or CDDO-TFEA at various concentrations (1,10 and 100nM in DMSO) for 18 hours and incubated with 2’,7’-Dichlorodihydrofluorecein diacetate (H2DCFDA) for 30 min.Cells are challenged with 250µM tBHP for 15-30 min and the mean fluorescence intensity for ~10,000 cells is analyzed by FACSan flow cytometry using a 480-nm excitation wavelength and a 525-nm emission wavelength. |
动物实验: |
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Mice G93A SOD1 transgenic familial ALS mice (high copy number) B6SJL background strain (G93A SOD1,B6SJL-TgGur1) are used.G93A transgenic mice are assigned randomly to the control (vehicle,mouse chaw only) and to mouse chaw containing either CDDO-EA or CDDO-TFEA (400mg/kg of food,n=30 in both groups).This dose corresponds to about 80mg/kg body weight/day,assuming each mouse consumes 5 grams of food per day.We found mice can tolerate this dose.Treatments started at two different time regimens:1) "Early" at 30 days of age,about two months prior to symptom onset;2) "At Onset" from the onset of the phenotype (80-90 days of age).A diet consisting of either 400mg of CDDO-TFEA per kg of food or 400mg of CDDO-EA per kg of food,and a control lab diet,are prepared by Purina. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )