中文名称: CDDO-EA
英文名称: CDDO-EA
CAS No: 932730-51-3
分子式: C33H46N2O3
分子量: 518.73
C11551 CDDO-EA ≥98% (psaitong)
包装规格:
5mg 10mg 50mg 100mg in glass bottle
溶解性:
溶于DMSO(≥30mg/mL)
产品描述:

基本信息

产品编号:

C11551

产品名称:

CDDO-EA

CAS:

932730-51-3

 

储存条件

粉末

-20℃

四年

 

 

分子式:

C33H46N2O3

溶于液体

-80℃

6个月

分子量:

518.73

-20℃

1个月

化学名: 

RTA 405;(4aS,6aR,6bS,8aR,12aS,14aR,14bS)-11-cyano-N-ethyl-2,2,6a,6b,9,9,12a-heptamethyl-10,14-dioxo-1,3,4,5,6,7,8,8a,14a,14b-decahydropicene-4a-carboxamide

Solubility (25°C):

 

体外:

 

DMSO

 

Ethanol

 

Water

 

体内(现配现用):

 

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

1.9278mL

9.6389mL

19.2779mL

5mM

0.3856mL

1.9278mL

3.8556mL

10mM

0.1928mL

0.9639mL

1.9278mL

 

生物活性

产品描述

一种NF-E2相关因子2/抗氧化反应元件(Nrf2/ARE)激活剂。

靶点

Nrf2/ARE

 

体外研究

CDDO-EA potently activates Nrf2/ARE in a cell culture model of ALS and in the G93A SOD1 mouse model of ALS.CDDO-EA is a potent inducer of apoptosis in A549 lung cancer cells,as shown both by PARP cleavage and Annexin staining.CDDO-EA is more potent than CDDO itself as inducers of heme oxygenase-1 (HO-1).In RAW264.7 macrophage-like cells,CDDO-EA is 7- fold more potent than CDDO as suppressors of the ability of IFN-γ to induce iNOS.

体内研究

The survival analysis shows that G93A mice treated with CDDO-EA,compared to G93A littermate controls, lives significantly longer.CDDO-EA treatment increases the life-span by 20.6 days from 124.05±3.7 days to 144.72±8.1 days (16.6%) (p<0.001).In CDDO-EA-treated G93A mice,the age of death is 141.4±5.2 days and the duration from the age of onset to the age of death is 57.6±7.6 days,which means that the age of death from onset is prolonged by 17.5 days (43%).

 

推荐实验方法(仅供参考)

细胞实验:

 

Wild-type and Nrf2−/−mouse embryonic fibroblasts are pre-treated with CDDO-EA or CDDO-TFEA at various concentrations (1,10 and 100nM in DMSO) for 18 hours and incubated with 2’,7’-Dichlorodihydrofluorecein diacetate (H2DCFDA) for 30 min.Cells are challenged with 250µM tBHP for 15-30 min and the mean fluorescence intensity for ~10,000 cells is analyzed by FACSan flow cytometry using a 480-nm excitation wavelength and a 525-nm emission wavelength.

 

动物实验:

 

Mice

G93A SOD1 transgenic familial ALS mice (high copy number) B6SJL background strain (G93A SOD1,B6SJL-TgGur1) are used.G93A transgenic mice are assigned randomly to the control (vehicle,mouse chaw only) and to mouse chaw containing either CDDO-EA or CDDO-TFEA (400mg/kg of food,n=30 in both groups).This dose corresponds to about 80mg/kg body weight/day,assuming each mouse consumes 5 grams of food per day.We found mice can tolerate this dose.Treatments started at two different time regimens:1) "Early" at 30 days of age,about two months prior to symptom onset;2) "At Onset" from the onset of the phenotype (80-90 days of age).A diet consisting of either 400mg of CDDO-TFEA per kg of food or 400mg of CDDO-EA per kg of food,and a control lab diet,are prepared by Purina.

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
搜索质检报告(COA)
参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • =
    *
    *
    *选择对应的单位 *空出希望得到的变量,填写另外两个变量

用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):