| 中文名称: | AZ505 | ||||
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| 英文名称: | AZ505 | ||||
| 别名: | N-环己基-3-(3,4-二氯苯乙基氨基)-N-[2-[[2-[5-羟基-3-氧代-3,4-二氢-2H-苯并[B][1,4]恶嗪-8-基]乙基]氨基]乙基]丙酰胺 N-cyclohexyl-3-[2-(3,4-dichlorophenyl)ethylamino]-N-[2-[2-(5-hydroxy-3-oxo-4H-1,4-benzoxazin-8-yl)ethylamino]ethyl]propanamide | ||||
| CAS No: | 1035227-43-0 | 分子式: | C29H38Cl2N4O4 | 分子量: | 577.54 |
| CAS No: | 1035227-43-0 | ||||
| 分子式: | C29H38Cl2N4O4 | ||||
| 分子量: | 577.54 | ||||
基本信息
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产品编号：A11400 |
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产品名称：AZ505 |
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CAS: |
1035227-43-0 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量： |
577.54 |
-20℃ |
一个月 |
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化学名： |
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Solubility (25°C) |
体外 |
DMSO |
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Ethanol |
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Water |
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体内（现配现用） |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
1.7315mL |
8.6574mL |
17.3148mL |
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5mM |
0.3463mL |
1.7315mL |
3.4630mL |
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10mM |
0.1731mL |
0.8657mL |
1.7315mL |
生物活性
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产品描述 |
一种有效的，具有选择性的 SMYD2 抑制剂，IC50 值为 0.12μM。 |
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靶点/IC50 |
0.12μM (SMYD2) |
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体外研究 |
AZ505 is highly selective and shows an activity at submicromolar concentrations in vitro. The IC50 of AZ505 for SMYD2 is 0.12μM, which is >600-fold greater than the IC50s of AZ505 for other histone methyltransferases, such as SMYD3 (IC50>83.3μM),DOT1L (IC50>83.3μM) and EZH2 (IC50>83.3μM). AZ505 is a potent and selective SMYD2 inhibitor with an IC50 of 0.12μM.The human SMYD (SET and MYND domain-containing protein) family of protein lysine methyltransferases contains five members (SMYD1-5). Moreover, AZ505 fails to inhibit the enzymatic activities of a panel of protein lysine methyltransferases.AZ505 is nominated for ITC binding study with Kd of 0.5μM. In contrast, the calculated Kd for the p53 substrate peptide is 3.7μM. AZ505 binding to SMYD2 is driven primarily by entropy, which often suggests that binding is mediated by hydrophobic interactions with few specific hydrogen bonds. |
推荐实验方法(仅供参考)
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激酶实验： |
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SMYD2 is expressed in insect cells and purified. AlphaScreen technology is used to screen our chemical library for small molecule inhibitors of SMYD2. Methylation (12μL) reactions are carried out in TDT buffer (50mM Tris-HCl [pH 9.0], 2mM DTT,and 0.01% Tween 20) at room temperature using 1.25nM SMYD2 protein, 200nM SAM, and 100 nM biotinylated p53 peptide substrate (Biotin-aminohexanoyl-GSRAHSSHLKSKKGQSTSRH) in low volume 384-well plates.Following a 75 min incubation period, reactions are quenched by the addition of 5μL of detection solution (20mM HEPES [pH 7.4], 1.7mg/mL BSA, 340mM NaCl, 680μM SAH,0.04mg/mL Streptavidin-coated AlphaScreen donor, and Protein A-coated acceptor beads),and 1nM of a custom p53K370me1 polyclonal antibody. Reaction plates are incubated overnight in the dark at room temperature, and read using an Envision 2101 Multi-label Reader.Compounds showing >50% inhibition of SMYD2 are nominated for concentration dose-response determination, and are also subjected to an artifact assay. Seven compound concentrations are selected beginning at 30μM with six half-log dilution steps. The artifact assay conditions are identical to those in the SMYD2 enzymatic activity assay, except for the absence of SMYD2 protein and the presence of 1 nM methylated p53 peptide.IC50 values are calculated from dose-response data using in-house software. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
