齐多呋定 - CAS:30516-87-1 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	齐多呋定促销
英文名称:	Zidovudine
别名:	叠氮胸苷 Azidothymidine; AZT; ZDV
CAS No:	30516-87-1	分子式:	C₁₀H₁₃N₅O₄	分子量:	267.24
CAS No:	30516-87-1
分子式:	C₁₀H₁₃N₅O₄
分子量:	267.24
MDL:	MFCD00006536

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF) 点击下载技术规格说明书(PDF)

Z10029 齐多呋定 ≥98% (psaitong)

相关产品

包装规格：

5g 25g 100g in glass bottle

溶解性：

溶于DMSO（≥ 100 mg/mL)

产品描述：

基本信息

产品编号：Z10029
产品名称：Zidovudine
CAS:	30516-87-1	储存条件	粉末	2-8℃	四年

分子式：	C10H13N5O4		溶于液体	-80℃	两年
分子量	267.24			-20℃	一个月
化学名：	1-((2R,4S,5S)-4-AZIDO-5-(HYDROXYMETHYL)TETRAHYDROFURAN-2-YL)-5-METHYLPYRIMIDINE-2,4(1H,3H)-DIONE
Solubility (25°C)	体外	DMSO	53mg/mL (198.32mM)
		Ethanol	53mg/mL warmed with 50ºC water bath (198.32mM)
		Water	26mg/mL warmed with 50ºC water bath (97.29mM)
	体内	现配现用
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	3.7420mL	18.7098mL	37.4195mL
5mM	0.7484mL	3.7420mL	7.4839mL
10mM	0.3742mL	1.8710mL	3.7420mL
50mM	0.0748mL	0.3742mL	0.7484mL

生物活性

产品描述	一种核苷逆转录酶抑制剂，有潜力用于 HIV 感染的研究。Zidovudine 增强 CRISPR/Cas9调节的编辑频率。
靶点/IC₅₀	Reverse transcriptase
体外研究	Zidovudine inhibits SVG, Primary human fetal astrocytes (PFA), peripheral blood mononuclear cells (PBMC), and monocytederived macrophages (MDM) with EC50 of 17, 1311, 8, and 5nM, respectively. Zidovudine inhibits SVG, PFA, PBMC, and MDM with EC90 of 0.205μM, 44.157μM, 0.481μM, and 0.219μM, respectively. Genome editing via CRISPR/Cas9 has become an efficient and reliable way to make precise, targeted changes to the genome of living cells. CXCR4 is a co-receptor for the human immunodeficiency virus type 1 (HIV-1) infection and has been considered as an important therapeutic target for AIDS. CXCR4 mediates viral entry into human CD4+ cells by binding to envelope protein, gp120. Human CXCR4 gene is efficiently disrupted by CRISPR/Cas9-mediated genome editing, leading to HIV-1 resistance of human primary CD4+ T cells.The Cas9-mediated ablation of CXCR4 demonstrated high specificity and negligible off-target effects without affecting celldivision and propagation.
体内研究	ntravitrous injection of the NRTIs Lamivudine (3TC), Zidovudine (AZT), or Abacavir (ABC) suppresses the laser-induced choroidal neovascularization (CNV) in wild-type mice compared to PBS vehicle. The mean level of VEGF-A in the RPE/choroid, which peaks on day 3 after laser injury, is significantly reduced in 3TC-, AZT- and ABC-treated eyes compared with control eyes in wild-type mice, but not inP2rx7-/- mice.
特征

推荐实验方法(仅供参考)

细胞实验:

Cell Assay

Assays are performed in all cell types in the presence of titrating concentrations of ARV. 5,000 SVG, 2,500 PFA, 200,000 PBMC,or 50,000 MDM cells/well are seeded into triplicate wells of 96-well plates. Twenty-four hours later, the culture medium is removed and replaced with medium containing the ARV or DMSO (0.5% vol/vol), and equivalent TCID50 infectious units of luciferase reporter virus are added to the cells. After a 16h incubation at 37°C, the initial viral inoculum is removed and replaced with culture medium containing the same antiretroviral drug (ARV) or DMSO (0.5% vol/vol) concentrations. At 72h post infection, the medium is aspirated, the cells are lysed and HIV-1 infection measured using the Luciferase Assay System. Luminescence is measured using a FLUOStar Optima microplate reader. Inhibition curves and the 50% (EC50) and 90% (EC90) effective concentrations are determined by nonlinear regression analysis, using GraphPad Prism software

动物实验:

Animal Administration

Mice C57BL/6J (wild-type) and P2rx7-/- mice are used. The Nlrp3-/- mice are used. The NRTIs 3TC, AZT, and ABC or the P2X7antagonist A438079 hydrochloride are dissolved in PBS. For CNV, each group of mice is injected once with 1μL of NRTIs (3TC, 125ng/μL; ABC, 183ng/μL; AZT, 146ng/μL), 1μL of A438079 hydrochloride (3, 30, or 300ng/μL), or the same volume of vehicle (PBS) into the vitreous humor using a 33-gauge needle immediately after laser injury. Another group of mice is injected with 3TC (125ng) in combination with an anti-mouse VEGF polyclonal antibody (10ng). Goat whole IgG (10ng) is used as a biological control for the anti-mouse VEGF antibody.

保存条件：

2-8℃

UN码：

HazardClass：

危害声明：

安全说明：

点击下载核磁/氢谱图谱(PDF)

点击下载高效液相/气相图谱(PDF)

搜索质检报告(COA)

参考文献 & 客户发表文献