| 中文名称: | XTT 钠盐 热销 | ||||
|---|---|---|---|---|---|
| 英文名称: | XTT sodium salt | ||||
| 别名: | 2,3-双(2-甲氧基-4-硝基-5-磺苯基)-2H-四氮唑-5-甲酰苯胺 内盐 Sodium 5,5'-(5-(phenylcarbamoyl)-1H-tetrazole-3-ium-2,3(3H)-diyl)bis(4-methoxy-2-nitrobenzenesulfonate) | ||||
| CAS No: | 111072-31-2 | 分子式: | C22H16N7NaO13S2 | 分子量: | 673.52 |
| CAS No: | 111072-31-2 | ||||
| 分子式: | C22H16N7NaO13S2 | ||||
| 分子量: | 673.52 | ||||
| MDL: | MFCD00083517 | ||||
一种四唑钠盐，可用于细胞存活率和细胞增殖的XTT测定，相对于MTT,具有更高的灵敏度和更大的动态范围。
XTT is a colorimetric assay used to assess cell viability as a function of cell number based on metabolic activity. This rapid, sensitive, non-radioactive assay is detected using standard microplate absorbance readers.
1.Grow cells in a 96-well plate at a density of 104–105 cells/well in 100 µL of culture medium with compounds to be tested. Culture in a CO2 incubator for 24–48 hours。
2.Make a 10 mM PMS solution in phosphate-buffered saline (3 mg PMS into 1 mL PBS)。PMS(psaitong P10018)
3.Dissolve 4 mg of XTT in 4 mL of 37°C cell culture medium。
4.Add 10 µL of the PMS solution the 4 mL of XTT solution created in step 3 immediately before labeling cells。
5.Add 25 µL of XTT/PMS solution directly to each well containing 100 µL cell culture。
6.Incubate for 2 hours at 37°C in a CO2 incubator。
7.Read absorbance at 450nm。
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
