中文名称: | Z-WEHD-FMK | ||||
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英文名称: | Z-WEHD-FMK | ||||
CAS No: | 210345-00-9 | 分子式: | C37H42FN7O10 | 分子量: | 763.77 |
CAS No: | 210345-00-9 | ||||
分子式: | C37H42FN7O10 | ||||
分子量: | 763.77 |
基本信息
产品编号:W10035 |
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产品名称:Z-WEHD-FMK |
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CAS: |
210345-00-9 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
763.77 |
-20℃ |
一个月 |
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化学名: |
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Solubility (25°C) |
体外 |
DMSO |
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Ethanol |
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Water |
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体内(现配现用) |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
1.3093mL |
6.5465mL |
13.0929mL |
5mM |
0.2619mL |
1.3093mL |
2.6186mL |
10mM |
0.1309mL |
0.6546mL |
1.3093mL |
生物活性
产品描述 |
一种强效具有细胞通透性可逆的 caspase-1/5 抑制剂。Z-WEHD-FMK 也抑制 cathepsin B 的活性 (IC50=6 μM)。Z-WEHD-FMK 可用于检测细胞凋亡。 |
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靶点/IC50 |
Caspase-1 |
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体外研究 |
WEHD-FMK (80μM; 9 hours) elicits a near-complete blockage of C.trachomatis-induced cleavage of golgin-84 and increases GM130 expression in cells.Z-WEHD-FMK (30min before being exposed to E. piscicida) effectively inhibits 0909I E. piscicida induced ZF4 cells cytotoxicity and pyroptotic morphology. And in addition, it also inhibits the cytotoxicity induced by cytosolic LPS delivery.Z-WEHD-FMK (20μM;18-24 hours following Cr3+,Ni2+, and Co2+) significantly induces a decrease of 76% to 86% in IL-1β release with 200 to 400 ppm Cr3+, it also induces a decrease of 35% to 45% with 48 ppm Ni2+ or higher, Finally, this caspase-1 inhibitor induced a decrease with 6 ppm Co2+, down to a level below the detection threshold, and a decrease of 40% to 48% with 12 to 24 ppm Co2+ in bone marrow-derived macrophages (BMDM). Western Blot Analysis |
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Cell Line: |
C. trachomatis- or mock-infected HeLa cells |
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Concentration: |
80μM |
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Incubation Time: |
9 hours |
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Result: |
Increased golgin-84 and GM130 expression. |
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Cell Viability Assay |
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Cell Line: |
Mycoplasma free-ZF4 cells |
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Concentration: |
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Incubation Time: |
30 min before being exposed to E. piscicida |
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Result: |
Inhibited ZF4 cells cytotoxicity and pyroptotic morphology. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )