| 中文名称: | trans-ACPD | ||||
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| 英文名称: | trans-ACPD | ||||
| 别名: | Trans-(±)-ACP | ||||
| CAS No: | 67684-64-4 | 分子式: | C7H11NO4 | 分子量: | 173.17 |
| CAS No: | 67684-64-4 | ||||
| 分子式: | C7H11NO4 | ||||
| 分子量: | 173.17 | ||||
基本信息
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产品编号： |
T70134 |
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产品名称： |
trans-ACPD |
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CAS: |
67684-64-4 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量 |
173.17 |
-20℃ |
1个月 |
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化学名： |
1-Amino-cyclopentane-1,3-dicarboxylic acid(trans-(1R,3R)-ACPD) |
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Solubility (25°C)： |
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体外:
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DMSO |
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Ethanol |
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Water |
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体内(现配现用): |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
5.7747mL |
28.8734mL |
57.7467mL |
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5mM |
1.1549mL |
5.7747mL |
11.5493mL |
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10mM |
0.5775mL |
2.8873mL |
5.7747mL |
生物活性
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产品描述 |
一种代谢型受体激动剂，促进钙离子活动及产生内向电流。 |
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靶点 |
mGluR |
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体外研究 |
Excitatory amino acid (EAA) analogues activate receptors that are coupled to the increased hydrolysis of phosphoinositides (PIs).In these studies,hippocampal slices are prepared from neonatal rats (6-11 days old) to characterize the effects of EAA analogues on these receptors.The concentrations of trans-ACPD required to evoke half-maximal stimulation (EC50 value) is 51μM.DL-2-Amino-3-phosphonopropionate (DL-AP3) is also equipotent as an inhibitor of PI hydrolysis stimulated by ibotenate,quisqualate,and trans-ACPD (IC50 values are 480-850μM). |
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体内研究 |
Intrathecal injection of NMDA,kainate,and trans-ACPD,TNF-α,or IL-1β causes significant (p<0.001) biting behaviour in mice compared to animals injected intrathecally with saline.In all groups,systemic pre-treatment with GM (100mg/kg,i.p.) significantly (p<0.001) reduces the biting behaviour compared to mice treated with saline (10mL/kg,i.p.). The greatest effect of GM is observed on the pro-inflammatory cytokines and NMDA,with the following inhibition percentages:TNF-α(92±7%),IL-1β(91±5%),NMDA (69±1%),and trans-ACPD (71±12%).By contrast,at the same dose,GM has no significant effect on the kainate-mediated biting response. |
推荐实验方法(仅供参考)
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动物实验: |
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Mice Male Swiss mice (25-35g) are used.Intrathecal injections are given to fully conscious mice.Briefly,the animals are manually restrained,and a 30-gauge needle connected by a polyethylene tube to a 25μL Hamilton gas-tight syringe is inserted through the skin and between the vertebrae into the subdural space of the L5-L6 spinal segments.Intrathecal injections (5μL/site) are administered over a period of 5 s.Biting behaviour is defined as a single head movement directed at the flanks or hind limbs,resulting in contact of the animal's snout with the target organ.The nociceptive response is elicited by NMDA (450 pmol/site,a selective agonist of the NMDA glutamatergic ionotropic receptor),kainate (110 pmol/site,a selective agonist of the kainate subtype of glutamatergic ionotropic receptors),and trans-ACPD (50nmol/site,a non-selective agonist of metabotropic glutamate receptors,which is active at group I and group II),TNF-α(0.1 pmol/site) and IL-1β(1 pmol/site) or saline (5μL/site, i.t.).The amount of time the animal spent biting or licking the caudal region is taken as evidence of nociception and is evaluated following local post injections of the following agonists:NMDA (5 min),kainate (4 min),and trans-ACPD TNF-α,and IL-1β(15 min).Animals received GM (100mg/kg,i.p.) 0.5h before intrathecal injection of 5μL of the drugs,while control animals received a similar volume of saline (10mL/kg, i.p.). |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
