中文名称: | 替诺福韦马来酸盐 | ||||
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英文名称: | Tenofovir maleate | ||||
别名: | GS 1278 maleate; PMPA maleate; TDF maleate | ||||
CAS No: | 1236287-04-9 | 分子式: | C13H18N5O8P | 分子量: | 403.28 |
CAS No: | 1236287-04-9 | ||||
分子式: | C13H18N5O8P | ||||
分子量: | 403.28 |
基本信息
产品编号:T10662 |
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产品名称:Tenofovir maleate |
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CAS: |
1236287-04-9 |
储存条件 |
粉末 |
-20℃ |
四年 |
分子式: |
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分子量 |
403.28 |
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化学名: |
[(2R)-1-(6-aminopurin-9-yl)propan-2-yl]oxymethylphosphonic acid;(Z)-but-2-enedioic acid |
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Solubility (25°C) |
体外 |
DMSO |
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Ethanol |
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Water |
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体内 |
现配现用 |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
生物活性
产品描述 |
一种核苷酸逆转录酶抑制剂,有潜力用于艾滋病毒和慢性乙型肝炎的研究。 |
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靶点/IC50 |
HIV |
Reverse Transcriptase |
体外研究 |
Tenofovir shows cytotoxic effects on cell viability in HK-2 cells, with IC50 values of 9.21 and 2.77μM at 48 and 72h in MTTassay, respectively. Tenofovir diminishes ATP levels in HK-2 cells. Tenofovir (3.0 to 28.8μM) increases oxidative stress andprotein carbonylation in HK-2 cells. Furthermore, Tenofovir induces apoptosis in HK-2 cells, and that apoptosis is inducedvia mitochondrial damage. Tenofovir and M48U1 formulated in 0.25% HEC each inhibits the replication of both R5-tropicHIV-1BaL and X4-tropic HIV-1IIIb in activated PBMCs, and inhibits several laboratory strains and patient-derived HIV-1isolates. The combined formulation of M48U1 and tenofovir in 0.25% HEC exhibits synergistic antiretroviral activity againstinfection with R5-tropic HIV-1BaL, and is not toxic to PBMCs. |
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体内研究 |
Tenofovir Disoproxil Fumarate (20, 50, 140, or 300mg/kg) administered to BLT mice, shows dose dependent activity duringvaginal HIV challenge in BLT humanized mice. Tenofovir Disoproxil Fumarate (50, 140, 300mg/kg) significantly reduces HIVtransmission in BLT mice. Tenofovir Disoproxil Fumarate (0.5, 1.5, or 5.0mg/kg/day, p.o.) induces a dose-dependentdecline in serum viremia in woodchucks chronically infected with WHV. Tenofovir Disoproxil Fumarate administration is safeand effective in the woodchuck model of chronic HBV infection. |
推荐实验方法(仅供参考)
细胞实验: |
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Cell Assay |
Cells are plated into 48-well tissue culture plates (39,000 cells/mL) and allowed to grow for 48 h followed by treatment withvehicle or Tenofovir. Following the treatment period, cell viability is assessed using the MTT assay. The MTT assay relies onthe conversion of tetrazolium dye 3-(4,5-dimethlthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to formazan byNAD(P)H-dependent oxidoreductases. |
动物实验: |
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Animal Administration |
Twenty adult chronic WHV carrier woodchucks are stratified equally by age, sex, body weight, and serum GGT activity intofive treatment groups consisting of four animals each: (i) Tenofovir Disoproxil Fumarate at 15.0 mg/kg once per day, (ii)Tenofovir Disoproxil Fumarate at 5.0mg/kg/day, (iii) Tenofovir Disoproxil Fumarate at 1.5mg/kg/day, (iv) TenofovirDisoproxil Fumarate at 0.5mg/kg/day, and (v) a placebo control. The woodchucks are treated daily for 4weeks andobserved for an additional 12weeks following cessation of drug treatment. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )