三乙烯硫代磷酰胺 - CAS:52-24-4 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	三乙烯硫代磷酰胺
英文名称:	Thio-TEPA
别名:	三乙烯硫代磷酰胺 Thio-TEPA
CAS No:	52-24-4	分子式:	C₆H₁₂N₃PS	分子量:	189.22
CAS No:	52-24-4
分子式:	C₆H₁₂N₃PS
分子量:	189.22
EINEC:	200-135-7
EINEC:	200-135-7

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T10556 三乙烯硫代磷酰胺 ≥98% (psaitong)

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包装规格：

100mg 250mg in glass bottle

溶解性：

溶于DMSO（≥ 50 mg/mL)

产品描述：

基本信息

产品编号：T10556
产品名称：Thio-TEPA
CAS:	52-24-4	储存条件	粉末	2-8℃	四年
分子式：	C6H12N3PS		溶于液体	-80℃	两年
分子量	189.22			-20℃	一个月
化学名：	Phosphorothioic triamide, N,N',N''-tri-1,2-ethanediyl-
Solubility (25°C)	体外	DMSO	100mg/mL (528.48mM)
		Ethanol	100mg/mL (528.48mM)
		Water	100mg/mL warmed with 50ºC water bath (528.48mM)
	体内	现配现用
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	5.2849mL	26.4243mL	52.8485mL
5mM	1.0570mL	5.2849mL	10.5697mL
10mM	0.5285mL	2.6424mL	5.2849mL
50mM	0.1057mL	0.5285mL	1.0570mL

生物活性

产品描述	一种 DNA 烷化剂，具有抗肿瘤作用。
靶点/IC₅₀	DNA Alkylator
体外研究	Thio-TEPA exhibits alkylating activity in rat liver slice incubation. Thio-TEPA does not affect the viability of rat liver slices,and is not accumulated in the slices at all doses of 2, 5, 10mM.
体内研究	Thio-TEPA (20mg/kg, i.p.) with total body irradiation (TBI) enhances donor-type blood chimerism during the first 10 weeks but is not dramatically higher than that of TBI group alone. Thio-TEPA alone improves both short- and long-term engraftment in mice.
特征

推荐实验方法(仅供参考)

细胞实验:

Cell Assay

The effect of Thio-TEPA on slice viability is studied by the determination of intracellular potassium (K+) leakage. Intracellular K+ is measured by flame photometry and is expressed in relation to the DNA content as measured using fluorometry. ThioTEPA is added at concentrations of 1, 2, 5, and 10mM and slices are incubated for 6, 12, and 24 h. The results are compared with the values found for drug-free controls. Thio-TEPA in medium and slices is measured following 3, 6, and 9 h incubation at the three highest concentrations. Krebs-HEPES buffer is used as the slicing buffer and Waymouth's MB 752/1 medium supplemented with 10μg gentamycin is used as the incubation medium.

动物实验:

Animal Administration

Mice

Male C57BL/6JIco mice, 19 weeks old, 25 to 30 g, are used as recipients. Congenic C57BL/6J-Gpi-1a/Gpi-1a (B6-Gpi-1a) and BALB.B10LiLa (Gpi-1a) mice are used as a source of syngeneic and allogeneic donor bone marrow, respectively. The latter combination is H-2-compatible (both H-2b), but mismatched on a number of minor histocompatibility loci. Thio-TEPA is dissolved in PBS and injected i.p. at a single bolus of 20mg/kg. Preliminary pilot experiments have established that this approximated to the maximal tolerated dose when higher doses result in lethal gastrointestinal toxicity. Cyclophosphamide (CY) is dissolved in PBS and administered i.p. at a single dose of 200mg/kg. Total body irradiation is delivered using a 137Cs γ-irradiation unit at a dose rate of 87cGy/min to a dose of 5Gy. After Thio-TEPA treatment the mice are maintained on neomycin (3.5g/L sterile drinking water) for 2weeks to minimize the possible problems of endotoxemia via gut toxicity.

保存条件：

2-8℃

UN码：

HazardClass：

危害声明：

安全说明：

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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果