着色剂-ALL - CAS:7423-31-6 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	着色剂-ALL 促销
英文名称:	Stains-All
别名:	着色剂-ALL 1-Ethyl-2-[(E)-3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide 3,3'-Diethyl-9-methyl-4,5,4',5'-dibenzothiacarbocyanine
CAS No:	7423-31-6	分子式:	C₂₆H₂₆N₄O₄S	分子量:	490.58
CAS No:	7423-31-6
分子式:	C₂₆H₂₆N₄O₄S
分子量:	490.58

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S70082 着色剂-ALL 96% (psaitong)

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包装规格：

250mg 1g in glass bottle

溶解性：

溶于DMSO（10mg/mL超声）

产品描述：

基本信息

产品编号：	S70082
产品名称：	Stains-All
CAS:	7423-31-6	储存条件	粉末	2-8℃(避光)	四年

分子式：	C26H26N4O4S		溶于液体	-80℃	6个月
分子量	490.58			-20℃	1个月
化学名：	1-Ethyl-2-[(E)-3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide 3,3'-Diethyl-9-methyl-4,5,4',5'-dibenzothiacarbocyanine
Solubility (25°C)：
体外:	DMSO	10 mg/mL (17.87mM; ultrasonic and warming and heat to 60°C)
	Water
	Ethanol
体内(现配现用):
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	1.7871mL	8.9353mL	17.8705mL
5mM	0.3574mL	1.7871mL	3.5741mL
10mM	0.1787mL	0.8935mL	1.7871mL

生物活性

产品描述	一种阳离子碳菁染料，可在RNA(蓝紫色)、DNA(蓝色)和蛋白质(红色)条带上染色。
靶点	Others
体外研究	Almost all of the proteins found in skeletal muscle extracts, including the Ca2++Mg2+-ATPase and the 53,000-Da glycoprotein of the sarcoplasmic reticulum, are stained red or pink with Stains-all. Calsequestrin, the 1 60,000-Da glycoprotein, and 170,000-Da protein are stained blue with Stains-all. The ratio of Stains-all staining (measured at 615nm) to that of Coomassie blue staining (measured at 575nm) is 1.3 for calsequestrin, 2.0 for calmodulin, 1.4 for troponin C, and 2.2 for S-100. Therefore, in addition to differentially staining these Ca2+-binding proteins blue, Stains-all is a more sensitive stain for these Ca2+-binding proteins than is Coomassie blue

推荐实验方法(仅供参考)

Kinase Assay

Slab gels are fixed overnight with 25% isopropyl alcohol and washed exhaustively in 25% isopropyl alcohol to remove SDS. The gels are then stained in the dark for at least 48 h with 0.0025% Stains-all, 25% isopropyl alcohol, 7.5% formamide, and 30mM Tris base, pH 8.8. The interaction of Stains-all with various Ca2+-binding proteins is also studied in aqueous solution. The standard solution contains 10mM Tris base, pH 8.8, 0.001% Stains-all, and 0.1% formamide. Ca2+-binding proteins (0.5 to 12μg) are added to 1.0mL of solution and then incubated at room temperature in the dark for 30 min. The absorbance at 600 nm is then measured against a control solution, containing no protein, using a spectrophotometer

保存条件：

2-8℃ 避光

UN码：

HazardClass：

危害声明：

安全说明：

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