中文名称: | 丽丝胺罗丹明B 钠盐 | ||||
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英文名称: | Sulforhodamine B sodium salt | ||||
别名: | 酸性红52 3520-42-1 | ||||
CAS No: | 3520-42-1 | 分子式: | C27H29N2NaO7S2 | 分子量: | 580.65 |
CAS No: | 3520-42-1 | ||||
分子式: | C27H29N2NaO7S2 | ||||
分子量: | 580.65 | ||||
EINEC: | 222-529-8 | ||||
EINEC: | 222-529-8 |
基本信息
产品编号: |
S11447 |
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产品名称: |
Sulforhodamine B sodium salt |
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CAS: |
3520-42-1 |
储存条件 |
粉末 |
室温 |
四年 |
分子式: |
溶于液体 |
-80℃(密封保存) |
6个月 |
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分子量: |
580.65 |
-20℃(密封保存) |
1个月 |
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化学名: |
sodium;4-[3-(diethylamino)-6-diethylazaniumylidenexanthen-9-yl]benzene-1,3-disulfonate |
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Solubility (25°C): |
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体外:
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DMSO |
Insoluble |
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Ethanol |
Insoluble |
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Water |
100mg/mL (172.22mM) |
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体内(现配现用): |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
1.7222mL |
8.6110mL |
17.2221mL |
5mM |
0.3444mL |
1.7222mL |
3.4444mL |
10mM |
0.1722mL |
0.8611mL |
1.7222mL |
生物活性
产品描述 |
荧光染色 |
靶点 |
Others |
体外研究 |
Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay).The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies.This method relies on the property of SRB,which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions;thus,the amount of bound dye can be used as a proxy for cell mass,which can then be extrapolated to measure cell proliferation.The protocol can be divided into four main steps:preparation of treatment,incubation of cells with treatment of choice,cell fixation and SRB staining, and absorbance measurement.This assay is limited to manual or semiautomatic screening,and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells.It also has applications in evaluating the effects of gene expression modulation (knockdown,gene expression upregulation),as well as to study the effects of miRNA replacement on cell proliferation. |
推荐实验方法(仅供参考)
细胞实验: |
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Gently add 25μL (96-well format) or 5μL (384-well format) cold 50% (wt/vol) TCA to each well directly to medium supernatant,and incubate the plates at 4℃ for 1h.Mixing is not required,as this could lead to some cells detaching from the bottom of the well.Wash the plates four times by submerging the plate in a tub with slow-running tap water and remove excess water by gently tapping the plate into a paper towel. After the last wash allow the plate to air-dry at room temperature. Add 50μL (96-well format) or 20μL (384-well format) of 0.04%(wt/vol) SRB solution to each well.Leave at room temperature for 1h and then quickly rinse the plates four times with 1% (vol/vol) acetic acid (200μL for 96-well format or 30μL for 384-well format) to remove unbound dye.Allow the plate to air-dry at room temperature. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )