| 中文名称: | SB-649868 | ||||
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| 英文名称: | SB-649868 | ||||
| 别名: | (S)-N-((1-(5-(4-氟苯基)-2-甲基噻唑-4-羰基)哌啶-2-基)甲基)苯并呋喃-4-甲酰胺 (S)-N-((1-(5-(4-Fluorophenyl)-2-methylthiazole-4-carbonyl)piperidin-2-yl)methyl)benzofuran-4-carboxamide | ||||
| CAS No: | 380899-24-1 | 分子式: | C26H24FN3O3S | 分子量: | 477.55 |
| CAS No: | 380899-24-1 | ||||
| 分子式: | C26H24FN3O3S | ||||
| 分子量: | 477.55 | ||||
基本信息
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产品编号： |
S11205 |
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产品名称： |
SB-649868 |
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CAS: |
380899-24-1 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量： |
477.55 |
-20℃ |
1个月 |
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化学名： |
(S)-N-((1-(5-(4-Fluorophenyl)-2-methylthiazole-4-carbonyl)piperidin-2-yl)methyl)benzofuran-4-carboxamide |
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Solubility (25°C)： |
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体外:
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DMSO |
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Ethanol |
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Water |
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体内(现配现用): |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
2.0940mL |
10.4701mL |
20.9402mL |
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5mM |
0.4188mL |
2.0940mL |
4.1880mL |
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10mM |
0.2094mL |
1.0470mL |
2.0940mL |
生物活性
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产品描述 |
一种口服有效的选择性食欲素 OX1 和 OX 2 受体拮抗剂。 |
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靶点 |
pKi:9.4 (OX1),9.5 (OX2) |
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体外研究 |
SB-649868 is identified as one the most in vitro potent dual OX1 and OX2 receptor antagonist known at that time (pKi=9.4 and 9.5 at the OX1 and OX2 receptor,respectively) .SB-649868 antagonizes orexin-A-induced inositol 1 phosphate (IP1) accumulation with the following pKB value (OX1=9.67;OX2=9.64).SB-649868 displaces the [3H]ACT-078573 receptor binding with the following pKi values:OX1=9.27;OX2=8.91.Increasing concentrations of SB-649868 (0.3nM-30nM) induces a rightward shift of the orexin-A CRCs with a depression of the agonist efficacy suggesting a clear non-surmountable behavior.The calculated apparent pKb values are 9.67±0.03 and 9.64±0.07 for OX1 and OX2. |
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体内研究 |
Pharmacokinetic studies in the male CD rat,performed at 1mg/kg,iv and 3mg/kg,po,demonstrate an excellent pharmacokinetic profile for a hypnotic agent featuring moderate clearance in plasma (Clp=24mL/min/kg), short half-life of (<0.6h) and a low volume of distribution (Vss=1.1l/kg),coupled with excellent oral bioavailability (F=85%) and good exposure in plasma (Cmax=333ng/mL).A brain to blood ratio (B/B) of 0.1:1 is observed 1h after iv administration,a value in line with the expected partition between the two compartments based on the lower tissue binding observed in vitro in brain tissues (fraction unbound/brain=5.28%) with respect to plasma proteins (fraction unbound/plasma=1.34%).SB-649868,administered orally 3h before OX-A injection at doses of 1,3 and 10mg/kg,causes a dose-dependent reduction of OX-A induced grooming as measured by total time spent grooming and number of grooming bouts (p<0.01 at 3 and 10mg/kg po).From dissociation kinetic studies using [3H]ACT-078573,the calculated long half-life,(t1/2) supports the nonsurmountability profile of SB-649868 (t1/2=35.91 min) at OX1 orexin receptor.The long or moderately long t1/2values for SB649868 at OX2 orexin receptor (t1/2=8.09 min). |
推荐实验方法(仅供参考)
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细胞实验: |
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Chinese Hamster Ovary (CHO) cells stably transfected with human OX1 orexin receptor are cultured in Dulbecco's modified Eagle's medium F12 Ham,supplemented with 10% fetal bovine serum (FBS),2mg/mL glutamine,600μg/ml geneticin at 37℃ in an atmosphere of 95% air and 5% CO2.CHO cells stably transfected with human OX2 orexin receptor are cultured in alpha-MEM supplemented with 10% FBS,100 units/mL penicillin G,100 units/mL streptomycin and 400μg/mL geneticin,at 37℃ in an atmosphere of 95% air and 5% CO2.Accumulation of IP1 is measured using IP-One HTRF terbium cryptate-based assay.OX1-CHO cells are seeded into white 384-well plate at the cell density of 1×104 cells per well and cultured for 24h in the presence of 5mM sodium butyrate while OX2-CHO cells are seeded at the cell density of 4×104 cells per well and cultured for 24h in culture medium.After washings Hank's Balanced Salt Solution (HBSS) at room temperature containing 20mM HEPES pH 7.4,50mM,LiCl and 0.1% Bovine Serum Albumin (BSA) cells are pre-incubated for 45 min with antagonist and then treated with agonist for 60 min at 37℃.Detection reagents,IP1-d2 tracer and anti-IP1-cryptate are diluted in lysis buffer and added to the cells.Following 60 min incubation at room temperature,time-resolved fluorescence at 615nm and 665nm are measured with Envision Multilabel flash lamp reader with 100 flashes and 400μs integration time. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
