| 中文名称: | SCR7 pyrazine 促销 | ||||
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| 英文名称: | SCR7 pyrazine | ||||
| 别名: | 2,3-Dihydro-6,7-diphenyl-2-thioxo-4(1H)-pteridinone | ||||
| CAS No: | 14892-97-8 | 分子式: | C18H12N4Os | 分子量: | 332.38 |
| CAS No: | 14892-97-8 | ||||
| 分子式: | C18H12N4Os | ||||
| 分子量: | 332.38 | ||||
| MDL: | MFCD02167478 | ||||
基本信息
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产品编号：S10075 |
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产品名称：SCR7 pyrazine |
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CAS: |
14892-97-8 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
两年 |
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分子量 |
332.38 |
-20℃ |
一个月 |
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化学名： |
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Solubility (25°C) |
体外 |
DMSO |
100mg/mL (300.86mM; Need ultrasonic) |
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Ethanol |
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Water |
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体内 |
现配现用 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
3.0086mL |
15.0430mL |
30.0860mL |
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5mM |
0.6017mL |
3.0086mL |
6.0172mL |
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10mM |
0.3009mL |
1.5043mL |
3.0086mL |
生物活性
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产品描述 |
一种NHEJ(非同源末端连接)抑制剂，增强CRISPR-CAS9介导的同源定向修复。 |
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靶点/IC50 |
DNA Ligase IV CRISPR/Cas9 |
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体外研究 |
SCR7 pyrazine (20-100μM; 24 hours; MCF7 cells) treatment interferes with NHEJ in cells, leading to accumulation of unrepaired double-strand breaks (DSBs). SCR7 pyrazine treatment shows a dose-dependent decrease in cell proliferation with IC50 values of 40μM, 34μM, 44μM, 8.5μM, 120μM, 10μM and 50μM for MCF7, A549, HeLa, T47D, A2780, HT1080 and Nalm6 cells, respectively.In MCF7 cells, SCR7 pyrazine (20, 40μM) treatment increases phosphorylation of ATM and activates p53, decreases MDM2,BCL2, resulting in activation of proapoptotic proteins, PUMA and BAX. And the shorter fragments of MCL1, PARP1, Caspase 3,and Caspase 9 cleavage are upregulated in a dose-dependent manner. Western Blot Analysis |
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Cell Line: |
MCF7 cells |
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Concentration: |
20μM, 40μM, 100μM |
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Incubation Time: |
24 hours |
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Result: |
Showed an increase in levels of gH2AX foci and protein. |
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体内研究 |
SCR7 pyrazine (10 mg/kg; intraperitoneal injection; six doses; BALB/c mice) treatment significantly reduces breast adenocarcinoma-induced tumor and increases lifespan . |
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Animal Model: |
BALB/c mice injected with breast adenocarcinoma cells |
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Dosage: |
10mg/kg |
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Administration: |
Intraperitoneal injection; on alternate days (0, 2, 4, 6, 8, and 10) |
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Result: |
Significantly reduced breast adenocarcinoma-induced tumor and increased lifespan. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
