| 中文名称: | RG14620 | ||||
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| 英文名称: | RG14620 | ||||
| 别名: | Tyrphostin RG14620 Tyrphostin RG14620 | ||||
| CAS No: | 136831-49-7 | 分子式: | C14H8Cl2N2 | 分子量: | 275.13 |
| CAS No: | 136831-49-7 | ||||
| 分子式: | C14H8Cl2N2 | ||||
| 分子量: | 275.13 | ||||
基本信息
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产品编号：R10442 |
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产品名称：RG14620 |
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CAS: |
136831-49-7 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
两年 |
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分子量 |
275.13 |
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化学名： |
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Solubility (25°C) |
体外 |
DMSO |
55 mg/mL (199.9 mM) |
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Ethanol |
6 mg/mL (21.8 mM) |
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Water |
Insoluble |
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体内 |
（现配现用） |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
3.6346mL |
18.1732mL |
36.3465mL |
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5mM |
0.7269mL |
3.6346mL |
7.2693mL |
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10mM |
0.3635mL |
1.8173mL |
3.6346mL |
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50mM |
0.0727mL |
0.3635mL |
0.7269mL |
生物活性
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产品描述 |
一种EGFR抑制剂；IC50值为3μM。 |
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靶点/IC50 |
EGFR |
ABCG2 |
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体外研究 |
RG14620 inhibits colony formation (IC50=3μM) and DNA synthesis (IC50=1μM) by HER 14 cells, which are stimulated by 50ng/mL EGF, in a dose-dependent manner. RG14620 also suppresses colony formation(IC50=4μM) and DNA synthesis (IC50 =1.25μM) by EGF-stimulated MH-85 cells in a dose-dependent manner. The growth-inhibitory effect of RG14620 irreversible |
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体内研究 |
RG14620, at a dose of 200g/mouse/day inhibits H-85 tumor growth in nude mice. Mice show less cachexia and hypercalcemia, eat more food, and are more active than untreated MH-85 tumor-bearing animals. |
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推荐实验方法(仅供参考)
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细胞实验: |
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MH-85 cells and HER 14 cells are plated in complete medium, either αMEM or DMEM, respectively, supplemented with 10% FCS. After overnight culture, the culture medium is switched to αMEM supplemented with 0.2% PCS and 50ng/mL EGF (MH85) or DMEM supplemented with 0.5% PCS and 50ng/mL EGF (HER14). The cells are cultured in this medium in the presence or absence of increasing concentrations of RG-13022 or RG-14620 for 10 days. At the end of culture, the cells are fixed with 4% (v/v) formaldehyde in calcium-magnesium-free phosphate-buffered saline for 15min at room temperature and stained with hematoxylin. Numbers of colonies including more than 20 cells in each well are counted under the microscope. |
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动物实验: |
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Mice: RG14620 in 0.1mL 100% DMSO is injected i.p. twice a day from 1day after MH-85 tumor inoculation. Control animals are given the same vehicle. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
