中文名称: | Pifithrin-μ 热销 | ||||
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英文名称: | Pifithrin-μ | ||||
别名: | 2-Phenyl-ethynesulfoanide | ||||
CAS No: | 64984-31-2 | 分子式: | C8H7NO2S | 分子量: | 181.21 |
CAS No: | 64984-31-2 | ||||
分子式: | C8H7NO2S | ||||
分子量: | 181.21 | ||||
MDL: | MFCD00181531 | EINEC: | 303580 | ||
EINEC: | 303580 |
基本信息
产品编号:P10061 |
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产品名称:Pifithrin-μ |
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CAS: |
64984-31-2 |
储存条件 |
粉末 |
20℃ |
四年 |
分子式: |
溶于液体 |
-80℃(存于氮气) |
六个月 |
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分子量 |
181.21 |
-20℃(存于氮气) |
一个月 |
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化学名: |
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Solubility (25°C) |
体外 |
DMSO |
36mg/mL (198.66mM) |
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Ethanol |
36mg/mL (198.66mM) |
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Water |
Insoluble |
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体内(现配现用) |
2% DMSO+30% PEG 300+ddH2O |
5mg/mL |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
5.5185mL |
27.5923mL |
55.1846mL |
5mM |
1.1037mL |
5.5185mL |
11.0369mL |
10mM |
0.5518mL |
2.7592mL |
5.5185mL |
50mM |
0.1104mL |
0.5518mL |
1.1037mL |
生物活性
产品描述 |
一种p53和HSP70的活性抑制剂。 |
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靶点/IC50 |
HSP70 |
MDM-2/p53 |
体外研究 |
Pifithrin-μ(10μM) is a p53 inhibitor, which inhibits p53 binding to mitochondria by reducing its affinity to antiapoptotic proteins Bcl-xL and Bcl-2 but has no effect on p53-dependent transactivation, activity of caspases 2, 8, 9 and 10 in a cell-free system, or NF-κB-dependent transcription[1]. Pifithrin-μ(PES) time- and dose-dependently reduces viability in A549 cells,with IC50s of 44.9 and 25.7µM at 24h and 48 h. Pifithrin-μ (20μM) suppresses the cell migration,induces cell cycle arrest and cell apoptosis in A549 and H460 cells. Pifithrin-μ (10 or 20µM) inhibits activities of AKT, ERK, and Hsp70 in A549 and H460cells. Pifithrin-μ(20µM) sensitizes A549 and H460 cell lines to TRAIL-induced cell proliferation inhibition and apoptosis. |
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体内研究 |
Pifithrin-μ (40mg/kg, i.p.) shows no protective effect against doses of radiation that cause gastrointestinal syndrome in mice. Pifithrin-μ(PES, 10mg/kg) shows antitumor effect in mice bearing A549 cells[2]. Pifithrin-μ exhibits neuroprotective effect with the P53-inhibitor pifithrin-μ after cardiac arrest in a rodent model. |
推荐实验方法(仅供参考)
细胞实验: |
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The cell viability is determined by the Cell Counting Kit-8 assay. Briefly, A549 and H460 cells are incubated in 96-well plates at a density of 5 × 103 per 100 µL of culture medium overnight. After treated with indicated concentration of Pifithrin-μ for 24 and 48h, 10µL of tetrazolium substrate are added to each well of the plate. After incubation at 37℃ for 1h, the absorbance is recorded at a wavelength of 450nm using a microplate reader. Each experiment is determined in triplicate and repeated at least three times. |
动物实验: |
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Mice A549 cells (1 × 107) are suspended in Matrigel and inoculated subcutaneously into the mice.Twelve mice bearing evident tumors are arbitrarily assigned to PBS control group and Pifithrin-μ treatment groups (six mice per group). When tumors reach a size of -5×5 mm2, mice are treated with either a single of intraperitoneal injection of Pifithrin-μ (20mg/kg) or PBS every two days. After 3-week treatment, mice are euthanized with carbon dioxide. Tumor burdens are evaluated by measuring body weight, tumor weight, and tumor volume. Tumor volume is determined as 0.5 × length × width2. Tumor samples are collected and fixed in 10% neutral buffered formalin. Hematoxylin and eosin staining and immunohistochemistry for histological analysis of tumor samples are measured. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )