中文名称: NCGC00244536
英文名称: NCGC00244536
CAS No: 2003260-55-5
分子式: C25H22N2O2
分子量: 382.45
N10664 NCGC00244536 ≥98% (psaitong)
包装规格:
5mg in glass bottle
溶解性:
溶于DMSO(36.67mg/mL 超声)
产品描述:

基本信息

产品编号:N10664

产品名称:NCGC00244536

CAS:

2003260-55-5

 

储存条件

粉末

-20℃

四年

 

 

分子式:

C25H22N2O2

溶于液体

-80℃

六个月

分子量:

382.45

-20℃

一个月

化学名: 

 

 

Solubility (25°C)

 

体外

DMSO

76mg/mL (198.71mM)

Ethanol

1.5mg/mL (3.92mM)

Water

Insoluble

体内(现配现用)

 

 

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

2.6147mL

13.0736mL

26.1472mL

5mM

0.5229mL

2.6147mL

5.2294mL

10mM

0.2615mL

1.3074mL

2.6147mL

50mM

0.0523mL

0.2615mL

0.5229mL

 

生物活性

产品描述

一种高效的 KDM4B 抑制剂,IC50 值为10nM。

靶点/IC50

KDM4

10nM

 

体外研究

NCGC00244536 displays high selectivity for the fast-growing AR-negative PC3 cells (IC50=40nM) and over 100-fold selectivity against the immortalized prostate epithelial cell lines PrEC1 and PrEC4. NCGC00244536 effectively inhibits AR-positive cell lines, including LNCaP and VCaP, with IC50s in the sub-micromolar range, and abolishes androgen-stimulated LNCaP cell growth.NCGC00244536 is also potent in inhibiting the growth of other cancer cell lines, including the breast cancer cell lines MDA-MB2 and MCF-7, with micromolar IC50s.

体内研究

Treatment with NCGC00244536 results in significant inhibition of tumor growth and animals do not exhibit any major toxicity and appear to be normal. Histological data clearly indicate that NCGC00244536-treated tumors have significant amount of cell apoptosis, necrosis, and fibrosis.

 

推荐实验方法(仅供参考)

细胞实验:

 

LNCaP cells are treated with 0.1, 0.2, 1, 2.5, 5, 20μM of NCGC00244536. Cell viability is measured using the MTT assay.

 

动物实验:

 

Mouse:For xenograft animal model, PC3 cell suspension is injected into 4-6 weeks old severe combined immunodeficient (SCID) mice. When tumors become palpable, animals are randomly grouped for drug treatment. Alzet osmotic minipump containing NCGC00244536 (20mg/kg)is subcutaneously inserted into each animal, which allowed continuous drug delivery to the tumor site for up to 5 days. Tumor volume is recorded every other days and calculated by using the ellipsoid formula.

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • =
    *
    *
    *选择对应的单位 *空出希望得到的变量,填写另外两个变量

用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):