中文名称: | NCGC00244536 | ||||
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英文名称: | NCGC00244536 | ||||
别名: | KDM4B Inhibitor B3 | ||||
CAS No: | 2003260-55-5 | 分子式: | C25H22N2O2 | 分子量: | 382.45 |
CAS No: | 2003260-55-5 | ||||
分子式: | C25H22N2O2 | ||||
分子量: | 382.45 |
基本信息
产品编号:N10664 |
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产品名称:NCGC00244536 |
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CAS: |
2003260-55-5 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
六个月 |
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分子量: |
382.45 |
-20℃ |
一个月 |
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化学名: |
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Solubility (25°C) |
体外 |
DMSO |
76mg/mL (198.71mM) |
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Ethanol |
1.5mg/mL (3.92mM) |
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Water |
Insoluble |
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体内(现配现用) |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
2.6147mL |
13.0736mL |
26.1472mL |
5mM |
0.5229mL |
2.6147mL |
5.2294mL |
10mM |
0.2615mL |
1.3074mL |
2.6147mL |
50mM |
0.0523mL |
0.2615mL |
0.5229mL |
生物活性
产品描述 |
一种高效的 KDM4B 抑制剂,IC50 值为10nM。 |
靶点/IC50 |
KDM4 |
10nM |
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体外研究 |
NCGC00244536 displays high selectivity for the fast-growing AR-negative PC3 cells (IC50=40nM) and over 100-fold selectivity against the immortalized prostate epithelial cell lines PrEC1 and PrEC4. NCGC00244536 effectively inhibits AR-positive cell lines, including LNCaP and VCaP, with IC50s in the sub-micromolar range, and abolishes androgen-stimulated LNCaP cell growth.NCGC00244536 is also potent in inhibiting the growth of other cancer cell lines, including the breast cancer cell lines MDA-MB2 and MCF-7, with micromolar IC50s. |
体内研究 |
Treatment with NCGC00244536 results in significant inhibition of tumor growth and animals do not exhibit any major toxicity and appear to be normal. Histological data clearly indicate that NCGC00244536-treated tumors have significant amount of cell apoptosis, necrosis, and fibrosis. |
推荐实验方法(仅供参考)
细胞实验: |
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LNCaP cells are treated with 0.1, 0.2, 1, 2.5, 5, 20μM of NCGC00244536. Cell viability is measured using the MTT assay. |
动物实验: |
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Mouse:For xenograft animal model, PC3 cell suspension is injected into 4-6 weeks old severe combined immunodeficient (SCID) mice. When tumors become palpable, animals are randomly grouped for drug treatment. Alzet osmotic minipump containing NCGC00244536 (20mg/kg)is subcutaneously inserted into each animal, which allowed continuous drug delivery to the tumor site for up to 5 days. Tumor volume is recorded every other days and calculated by using the ellipsoid formula. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )