NI-57 - CAS:1883548-89-7 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	NI-57
英文名称:	NI-57
CAS No:	1883548-89-7	分子式:	C₁₉H₁₇N₃O₄S	分子量:	383.42
CAS No:	1883548-89-7
分子式:	C₁₉H₁₇N₃O₄S
分子量:	383.42

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N10663 NI-57 ≥98% (psaitong)

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包装规格：

50mg in glass bottle

溶解性：

溶于DMSO（100mg/mL 超声）

产品描述：

基本信息

产品编号：N10663
产品名称：NI-57
CAS:	1883548-89-7	储存条件	粉末	-20℃	四年

分子式：	C19H17N3O4S		溶于液体	-80℃	六个月
分子量：	383.42			-20℃	一个月
化学名：
Solubility (25°C)	体外	DMSO	'mg/mL
		Ethanol
		Water
	体内（现配现用）
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.6081mL	13.0405mL	26.0811mL
5mM	0.5216mL	2.6081mL	5.2162mL
10mM	0.2608mL	1.3041mL	2.6081mL

生物活性

产品描述	一种溴结构域和植物同源锌指结构域 (BRPF) 蛋白家族的抑制剂，对 BRPF1，BRPF2 (BRD1) 和 BRPF3 的 IC50 值分别为 3.1，46 和 140nM。
靶点/IC50	3.1nM (BRPF1),46nM (BRPF2 (BRD1)),140nM (BRPF3)
体外研究	NI-57 is an inhibitor of bromodomain and plant homeodomain finger-containing (BRPF), with IC50s of 3.1, 46 and 140nM for BRPF1, BRPF2 (BRD1) and BRPF3,respectively. NI-57 binds the BRD of BRPF1 with a Kd of 31 ± 2nM, BRD1 with a Kd of 110±13nM, and BRPF3 with a Kd of 410±47nM, whereas binding to BRD9 is weaker (Kd 1000±130nM) measured by isothermal titration calorimetry.NI-57 shows less active effect on BRD9 (IC50, 520nM) and BRD4 (BD1 (IC50,3700nM), TRIM24 (IC50,1600nM). NI-57 also inhibits BRPF BRDs in the nucleus, but shows little effect on the proliferation of many cancer cell lines with GI50s of 10.4μM (NCI-H1703 cells),14.7μM (DMS114), 15.6μM (HRA-19), and 16.6μM (RERF-LC-Sq1). Furthermore,Inhibition on BRPF1 of NI-57 (10μM) reduces the gene expression of CCL-22 by 27.7 ± 9.4%.
体内研究	NI-57 has favorable oral bioavailability in mice.

推荐实验方法(仅供参考)

激酶实验：

All reagents are diluted in the recommended buffer (50mMHEPES, 100mM NaCl, 0.1% BSA;pH=7.4) supplemented with 0.05% CHAPS and allowed to equilibrate to room temperature prior to addition to plates. 4mL of HIS-tagged protein is added to low-volume 384-well plates,followed by 4mL of either buffer, non-biotinylated peptide, solvent or compounds (NI-57,etc.). Plates are sealed and incubated at room temperature for 30 minutes, before the addition of 4mL biotinylated peptide, resealing and incubation for a further 30 minutes. 4mL of streptavidin-coated donor beads (25µg/mL) and 4 µL of nickel chelate acceptor beads (25µg/mL) are then added under low light conditions. Plates are foil sealed to protect from light, incubated at room temperature for 60 minutes and read on a PHERAstar FS plate reader using an AlphaScreenTM 680 excitation/570 emission filter set. IC50s are calculated in GraphPad Prism 5.Results for compounds (NI-57, etc.) dissolved in DMSO are normalised against corresponding DMSO controls prior to IC50 determination, which are given as the final concentration of compound in the 20µL reaction volume.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果