中文名称: NSC745885
英文名称: NSC745885
CAS No: 4219-52-7
分子式: C14H6N2O2S
分子量: 266.27
N10502 NSC745885 ≥98% (psaitong)
产品描述:

基本信息

产品编号:N10502

产品名称:NSC745885

CAS:

4219-52-7

 

储存条件

粉末

-20℃

四年

 

 

分子式:

C14H6N2O2S

溶于液体

-80℃

六个月

分子量

266.27

-20℃

一个月

化学名: 

 

 

Solubility (25°C)

 

体外

DMSO

 

Ethanol

 

Water

 

体内(现配现用)

 

 

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

生物活性

产品描述

一种有效的抗肿瘤 (anti-tumor) 试剂,对多种癌细胞株有选择性毒性,但对正常细胞没有毒性。

靶点/IC50

EZH2

体外研究

NSC745885 (0.5-4μM;24,48 or 72 hours) has a growth inhibitory or death-promoting effect on the SAS cells,it significantly decreases the densities of cultured cells when compared with untreated cells. The IC50 of NSC745885 is 0.85μM after 72 hours’treatment. NSC745885 (0.5-4μM; 24 hours) increases annexin V positive cells in a dose-dependent manner, and the differences appears as a dose-dependent manner. NSC745885 (0.5-2μM; 24 or 48 hours) decreases XIAP protein levels and increases protein levels both as a dose-dependent manner in SAS cells.
Cell Viability Assay

Cell Line:

SAS cells is obtained from a poorly differentiated human squamous cell carcinoma

Concentration:

0.5μM,1μM,1.5μM,2μM,4μM

Incubation Time:

24,48,or 72 hours

Result:

Decreases SAS cells growth as a time and dose-dependent manner.

Apoptosis Analysis

Cell Line:

SAS cells is obtained from a poorly differentiated human squamous cell carcinoma

Concentration:

0.5μM,1μM,1.5μM,2μM,4μM

Incubation Time:

24 hours

Result:

 

Decreases SAS cells growth as a time and dose-dependent manner.

Western Blot Analysis

Cell Line:

SAS cells is obtained from a poorly differentiated human squamous cell carcinoma

Concentration:

0.5μM,1μM,1.5μM,2μM

Incubation Time:

24 or 48 hours

Result:

 

Increased cleaved caspase-3 expression and decreased XIAP expression.

体内研究

NSC745885 (intraperitoneal injection; 2mg/kg; once daily; 10 days) treatment significantly reduces tumor size when compared with the vehicle control, and exhibits a higher safety than doxorubicin.

Animal Model:

Eight-week-old NOD/SCID (NOD.CB17 Prkdcscid/J) mice

Dosage:

2mg/kg

Administration:

Intraperitoneal injection;2mg/kg;once daily;10 days

Result:

Inhibited engrafted tumors growth in vivo.

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
搜索质检报告(COA)
参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

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用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

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连续稀释计算器方程

  • 连续稀释

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  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):