NITD008 - CAS:1044589-82-3 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	NITD008
英文名称:	NITD008
别名:	7-Deaza-2'-C-acetylene-adenosine
CAS No:	1044589-82-3	分子式:	C₁₃H₁₄N₄O₄	分子量:	290.27
CAS No:	1044589-82-3
分子式:	C₁₃H₁₄N₄O₄
分子量:	290.27

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF)

N10445 NITD008 ≥98% (psaitong)

相关产品

包装规格：

1mg 5mg in glass bottle

溶解性：

溶于DMSO(≥ 50 mg/mL)

产品描述：

基本信息

产品编号：N10445
产品名称：NITD008
CAS:	1044589-82-3	储存条件	粉末	-20℃	四年

分子式：	C13H14N4O4		溶于液体	-80℃	六个月
分子量	290.27			-20℃	一个月
化学名：	(2R,3R,4R,5R)-2-(4-aminopyrrolo[2,3-d]pyrimidin-7-yl)-3-ethynyl-5-(hydroxymethyl)oxolane-3,4-diol
Solubility (25°C)	体外	DMSO	≥50mg/mL (172.25mM)
		Ethanol
		Water
	体内	现配现用
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	3.4451mL	17.2253mL	34.4507mL
5mM	0.6890mL	3.4451mL	6.8901mL
10mM	0.3445mL	1.7225mL	3.4451mL

生物活性

产品描述	一种有效的和选择性的黄病毒 (flaviviruses) 抑制剂，抑制登革热病毒2型 (Dengue Virus Type 2 (DENV-2)) 的 EC50 值为0.64μM。
靶点/IC₅₀	EC50: 0.64μM (DENV-2)
体外研究	NITD008 potently inhibits other, including Dengue virus (DENV), West Nile virus, yellow fever virus, and Poissan virus. NITD008 inhibits DENV-2 in a dose-responsive manner, with an EC50 value of 0.64μM; treatment with 9μM compound reduces viral titer by >104-fold. NITD008 also inhibits a luciferase-reporting replicon of hepatitis C virus (HCV, genotype 1b), a member from the genus Hepacivirus, with an EC50 value of 0.11μM.
体内研究	NITD008 is orally bioavailable and has good pharmacokinetic properties. NITD008 exhibits the best pharmacokinetic parameters when formulated using 6 N of HCl (1.5 equimolar amount), 1 N of NaOH (pH adjusted to 3.5), and 100mM citrate buffer (pH 3.5). Following i.v. injection, NITD008 has a high volume of distribution (3.71 L/kg) and a low systemic clearance(31.11mL/min per kg), resulting in a long elimination half-life (t1/2=4.99 h). After p.o. dosing, NITD008 is rapidly absorbed(time of peak plasma concentration=0.5 h), with a maximal plasma concentration of 3μM and bioavailability of 48%. Treatment of the mice immediately after viral infection with 1 mg/kg of NITD008 does not reduce mortality, but treatment with 3 mg/kg partially protects and treatment with ≥10mg/kg completely protects the infected mice from death. NITD008 can suppress peak viremia, decrease cytokine elevation, and prevent death.

推荐实验方法(仅供参考)

细胞实验：
Cell Assay	For measurement of compound cytotoxicity, Vero cells (10000 cells per well of a 96-well plate) are incubated with various concentrations of NITD008 (3, 6, 12, 25, 50μM) for 48 h; cell viability is quantified using a MTT assay .

动物实验:

Animal Administration

Mice

The in vivo efficacy of NITD008 is evaluated in a dengue viremia model and a lethal model in mice. Both models use AG129 mice (with knockout IFN-α/β and IFN-γ receptors). DENV-2 strains TSV01and D2S10, respectively, are used in the 2 models and are propagated in C6/36 mosquito cells grown in RPMI-1640 medium with 5% FBS (vol/vol) at 28°C. The evaluation in the lethal model is performed by injecting mice i.v. with 0.2mL of RPMI-1640 medium containing 3×10⁷pfu/mL DENV-2 strain D2S10; the infected mice are then subjected to different treatment regimens, as indicated in each experiment. NITD008 (1, 3, 10, 25, 50mg/kg) in 0.2-0.25mL of formulation solution is administered by p.o. gavage. The mice (6 or 8 mice per group) are monitored twice a day.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果