2-硝基苯基-β-D-吡喃半乳糖苷 - CAS:369-07-3 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	2-硝基苯基-β-D-吡喃半乳糖苷促销
英文名称:	2-Nitrophenyl β-D-galactopyranoside
别名:	2-硝基苯基-β-D-吡喃半乳糖苷 ONPG
CAS No:	369-07-3	分子式:	C₁₂H₁₅NO₈	分子量:	301.25
CAS No:	369-07-3
分子式:	C₁₂H₁₅NO₈
分子量:	301.25
MDL:	MFCD00063255	EINEC:	206-716-1
EINEC:	206-716-1

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N10033 2-硝基苯基-β-D-吡喃半乳糖苷 ≥99% (psaitong)

相关产品

熔点：

180-193°C

包装规格：

1g 5g 25g in poly bottle

溶解性：

溶于水（3mg/mL 加热）

产品描述：

基本信息

产品编号：	N10033
产品名称：	2-Nitrophenyl β-D-galactopyranoside
CAS:	369-07-3	储存条件	粉末	-20℃	四年

分子式：	C12H15NO8		溶于液体	-80℃	六个月
分子量	301.25			-20℃	一个月
化学名：	(2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(2-nitrophenoxy)tetrahydro-2H-pyran-3,4,5-triol
Solubility (25°C)：
体外:	DMSO
	Ethanol
	Water	5mg/mL (16.5975mM; ultrasonic and warming and heat to 60°C)
体内(现配现用):
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	3.3195mL	16.5975mL	33.1950mL
5mM	0.6639mL	3.3195mL	6.6390mL
10mM	0.3320mL	1.6598mL	3.3195mL

生物活性

产品描述

一种β-半乳糖苷酶的比色底物。用于比色和EIA的应用，是广泛使用的PNPP/碱性磷酸酶底物的对应物。以硝基苯酚为最终产物，在405nm处进行监测。

体外研究

The enzyme displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity. To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8 s^-1 and 2.7±0.3mM for ONPG, and 264.2±2.1 s^-1 and 7.1±0.8mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 s^-1mM^-1) is 4.6-fold higher than that for lactose (37.2 s^-1mM^-1), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose

推荐实验方法(仅供参考)

Kinase Assay

The β-galactosidase activity is measured using two substrates including ONPG and lactose in this study. The β-galactosidase activity for ONPG is measured by following the amount o-nitrophenol released from ONPG. The reaction mixture is composed of 100μL of the enzyme solution and 400μL of ONPG solution (2.5 g/L in 100mM Tris-HCl buffer at pH 6.8). After incubation at 78°C for 15 min, the reaction is terminated by adding an equal volume of 1 M Na2CO3. The released onitrophenol is quantitatively determined by measuring at A₄₀₅. One unit of activity is defined as the amount of enzyme needed to produce 1μmol of o-nitrophenol per minute under the assay condition. The specific activity is expressed as units per milligram of protein. Assays for activity towards lactose are performed in the same buffer containing 100μL of enzyme solution and 5% lactose, and the reaction is stopped by boiling for 10 min, and the concentration of glucose is determined using a glucose oxidase-peroxidase assay kit. The released glucose is quantitatively determined by measuring A₄₉₂. One unit of enzyme activity is defined as the amount of activity required to release 1μmol of glucose per minute

保存条件：

-20℃

UN码：

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