中文名称: ML-18
英文名称: ML-18
CAS No: 1422269-30-4
分子式: C32H35N5O5
分子量: 569.65
M10924 ML-18 ≥98% (psaitong)
包装规格:
100mg in glass bottle
溶解性:
溶于DMSO(≥100mg/mL )
产品描述:

基本信息

产品编号:

M10924

产品名称:

ML-18

CAS:

1422269-30-4

 

储存条件

粉末

-20℃

四年

 

 

分子式:

C32H35N5O5

溶于液体

-80℃

6个月

分子量:

569.65

-20℃

1个月

化学名: 

(2S)-3-(1H-indol-3-yl)-N-[[1-(4-methoxyphenyl)cyclohexyl]methyl]-2-[(4-nitrophenyl)carbamoylamino]propanamide

Solubility (25°C):

 

体外:

 

DMSO

 

Ethanol

' mg/mL

Water

 

体内(现配现用):

 

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

1.7555mL

8.7773mL

17.5546mL

5mM

0.3511mL

1.7555mL

3.5109mL

10mM

0.1755mL

0.8777mL

1.7555mL

 

生物活性

产品描述

一种抑制肺癌生长的非肽铃蟾肽受体亚型-3 (BRS-3) 拮抗剂。

靶点

IC50:4.8μM (BRS-3)

 

体外研究

ML-18 inhibits specific 125I-BA1 (DTyr-Gln-Trp-Ala-Val-βAla-His-Phe-Nle-NH2)BB6-14 binding to NCI-H1299 lung cancer cells stably transfected with BRS-3 with IC50 values of 4.8μM.ML-18 binds with lower affinity to the GRPR and NMBR with IC50 values of 16 and more than 100μM,respectively.ML-18 at 16μM inhibits the ability of 10nM BA1 to elevate cytosolic Ca2+in a reversible manner using lung cancer cells loaded with FURA2-AM.ML-18 at 16μM inhibits the ability of 100nM BA1 to cause tyrosine phosphorylation of the EGFR and ERK in lung cancer cells.It inhibits the proliferation of lung cancer cells.

 

推荐实验方法(仅供参考)

激酶实验:

The cells are incubated in SIT buffer containing 0.25% bovine serum albumin and 250μg/mL bacitracin and 125I-BA1 (100,000 cpm) is added,as well as various concentrations of unlabelled competitor (ML-18). After incubation at 37℃ for 30 min,free 125I-BA1 is removed by washing 3 times in buffer and the cells which contain bound 125I-BA1 is dissolved in 0.2N NaOH and counted in a gamma counter.The IC50 is calculated for each unlabeled competitor.

 

细胞实验:

 

Cell viability is measured using the MTT assay.NCI-H727 or NCI-H1299 cells transfected with BRS-3 are treated with ML-18 (0,4.8,16,48μM) or gefitinib added.After 2 days,15μL of 0.1 % MTT solution added.After 4h,150μL of DMSO is added.After 16h,the optical density at 570nm is determined.

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

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用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

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  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):