| 中文名称: | MM-102 TFA | ||||
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| 英文名称: | MM-102 TFA | ||||
| 别名: | HMTase Inhibitor IX TFA | ||||
| CAS No: | 1883545-52-5 | 分子式: | C37H50F5N7O6 | 分子量: | 783.83 |
| CAS No: | 1883545-52-5 | ||||
| 分子式: | C37H50F5N7O6 | ||||
| 分子量: | 783.83 | ||||
基本信息
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产品编号：M10871 |
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产品名称：MM-102 TFA |
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CAS: |
1883545-52-5 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量： |
783.83 |
-20℃ |
一个月 |
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化学名： |
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Solubility (25°C) |
体外 |
DMSO |
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Ethanol |
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Water |
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体内（现配现用） |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
1.2758mL |
6.3789mL |
12.7579mL |
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5mM |
0.2552mL |
1.2758mL |
2.5516mL |
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10mM |
0.1276mL |
0.6379mL |
1.2758mL |
生物活性
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产品描述 |
一种有效的 WDR5/MLL 相互作用抑制剂, WDR5 结合检测中, IC50 =2.4nM, Ki< 1nM。 |
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靶点/IC50 |
2.4nM (MLL). |
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体外研究 |
MM-102 (HMTase Inhibitor IX) inhibits MLL1 methyltransferase activity and MLL-1-induced HoxA9 and Meis-1 gene expression in leukemia cells expressing the MLL1-AF9 fusion gene. Also inhibits cell growth and induces apoptosis in leukemia cells harbouring MLL1 fusion proteins.MM-102 (TFA), with the highest binding affinities to WDR5, also show the most potent inhibitory activity in the HMT assay withIC50=0.4-0.9μM.MM-102 (HMTase Inhibitor IX) dose-dependently inhibits cell growth in the MV4;11 and KOPN8 leukemia cell lines, which carry MLL1-AF4 and MLL1-ENL fusion proteins, respectively.MM-102 (HMTase Inhibitor IX) has IC50=25μM in both cell lines and completely inhibits cell growth in these cell lines at 75μM.MM-102 (HMTase Inhibitor IX) effectively and selectively inhibits cell growth and induces apoptosis in leukemia cells harboring MLL1 fusion proteins and has minimal effect in leukemia cells with wild-type MLL1 protein. |
推荐实验方法(仅供参考)
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细胞实验: |
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MV4;11, KOPN8, and K562 cells were cultured in RPMI 1640 medium (ATCC) supplemented with 10% fetal bovine serum and 100 U/L penicillinstreptomycin and incubated at 37℃under 5% CO2.Cells were seeded into 12-well plates for suspension at a density of 5×105 per well (1mL) and treated with either vehicle control (DMSO, 0.2%) or MM-102 (HMTase Inhibitor IX) for 7 days.The medium was changed every 2 days, and compounds were resupplied. The CellTiter-Glo Luminescent Cell Viability Assay kit was used. First, 100μL of the assay reagent was added into each well, and the content was mixed for 2 min on an orbital shaker to induce cell lysis. After 10 min incubation at room temperature, the luminescence was read on a microplate reader. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
