中文名称: MT 63-78
英文名称: MT 63-78
CAS No: 1179347-65-9
分子式: C21H14N2O2
分子量: 326.35
M10799 MT 63-78 (psaitong)
包装规格:
5mg 10mg 25mg 50mg in glass bottle
产品描述:

基本信息

产品编号:M10799

产品名称:MT 63-78

CAS:

1179347-65-9

 

储存条件

粉末

-20℃

四年

分子式:

C21H14N2O2

溶于液体

-80℃(避光)

六个月

分子量:

326.35

-20℃(避光)

一个月

化学名: 

 

 

Solubility (25°C)

 

体外

DMSO

 

Ethanol

 

Water

 

体内(现配现用)

 

 

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

3.0642mL

15.3210mL

30.6419mL

5mM

0.6128mL

3.0642mL

6.1284mL

10mM

0.3064mL

1.5321mL

3.0642mL

 

生物活性

产品描述

一种有效的直接 AMPK 激活剂,EC50 为 25μM。M 63-78 还诱导细胞有丝分裂阻滞和细胞凋亡 (apoptosis)。

靶点/IC50

AMPK

25μM (EC50)

mTORC1

 

体外研究

MT 63-78 (0-50μM;4 days;LNCaP and PC3 cells) treatment shows a dose-dependent decrease in cell number,and concomitant to the activation of AMPK signaling.MT 63-78 (25μM;24 hours;LNCaP and CRPC cells) treatment induces a significant enrichement in the G2/M population.MT 63-78 (0-50μM;24 hours;LNCaP,PC3,C4-4,C4-2B,CL1and 22RV1cells) treatment induces reduction of anti-apoptotic Mcl-1 in concert with accumulation of the pro-apoptotic BH3-only protein Puma.MT 63-78 (0-50μM;30 minutes;LNCaP and PC3 cells) treatment shows a dose-dependent phosphorylation of the two major AMPK targets Acetyl-CoA Carboxylase (ACC) on Ser79 and of Raptor on Ser792.And also increases Thr172 phosphorylation on the AMPK α subunit.

Cell Viability Assay

Cell Line:

LNCaP and PC3 cells

Concentration:

0μM,1μM,5μM,10μM,25μM,50μM

Incubation Time:

4 days

Result:

A dose-dependent decrease in cell numberconcomitant to the activation of AMPK signaling was observed.

Cell Cycle Analysis

Cell Line:

LNCaP and CRPC cells

Concentration:

25μM

Incubation Time:

24 hours

Result:

Induced a significant enrichement in the G2/M population in both androgen sensitive and CRPC cell models.

Apoptosis Analysis

Cell Line:

LNCaP, PC3, C4-4, C4-2B, CL1and 22RV1cells

Concentration:

0μM,10μM,25μM,50μM

Incubation Time:

24 hours

Result:

Induced reduction of anti-apoptotic Mcl-1 in concert with accumulation of the proapoptotic BH3-only protein Puma in all PCa cells.

Western Blot Analysis

Cell Line:

LNCaP and PC3 cells

Concentration:

0μM,0.25μM,0.5μM,1μM,5μM,25μM,50μM

Incubation Time:

30 minutes

Result:

Observed a dose-dependent phosphorylation of the two major AMPK targets Acetyl-CoA Carboxylase (ACC) on Ser79 and of Raptor on Ser792.A corresponding increase in Thr172 phosphorylation on the AMPK α subunit was also observed.

体内研究

MT 63-78 (30mg/kg;intraperitoneal injection;daily;for 14 days;C57 BL/6 male mice) treatment leads to a 33% inhibition of tumor growth.

Animal Model:

C57 BL/6 male mice bearing LNCaP tumors

Dosage:

30mg/kg

Administration:

Intraperitoneal injection;daily;for 14 days

Result:

Led to a 33% inhibition of tumor growth.

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • =
    *
    *
    *选择对应的单位 *空出希望得到的变量,填写另外两个变量

用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):