中文名称: | MT 63-78 | ||||
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英文名称: | MT 63-78 | ||||
CAS No: | 1179347-65-9 | 分子式: | C21H14N2O2 | 分子量: | 326.35 |
CAS No: | 1179347-65-9 | ||||
分子式: | C21H14N2O2 | ||||
分子量: | 326.35 |
基本信息
产品编号:M10799 |
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产品名称:MT 63-78 |
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CAS: |
1179347-65-9 |
储存条件 |
粉末 |
-20℃ |
四年 |
分子式: |
溶于液体 |
-80℃(避光) |
六个月 |
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分子量: |
326.35 |
-20℃(避光) |
一个月 |
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化学名: |
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Solubility (25°C) |
体外 |
DMSO |
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Ethanol |
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Water |
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体内(现配现用) |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
3.0642mL |
15.3210mL |
30.6419mL |
5mM |
0.6128mL |
3.0642mL |
6.1284mL |
10mM |
0.3064mL |
1.5321mL |
3.0642mL |
生物活性
产品描述 |
一种有效的直接 AMPK 激活剂,EC50 为 25μM。M 63-78 还诱导细胞有丝分裂阻滞和细胞凋亡 (apoptosis)。 |
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靶点/IC50 |
AMPK 25μM (EC50) |
mTORC1 |
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体外研究 |
MT 63-78 (0-50μM;4 days;LNCaP and PC3 cells) treatment shows a dose-dependent decrease in cell number,and concomitant to the activation of AMPK signaling.MT 63-78 (25μM;24 hours;LNCaP and CRPC cells) treatment induces a significant enrichement in the G2/M population.MT 63-78 (0-50μM;24 hours;LNCaP,PC3,C4-4,C4-2B,CL1and 22RV1cells) treatment induces reduction of anti-apoptotic Mcl-1 in concert with accumulation of the pro-apoptotic BH3-only protein Puma.MT 63-78 (0-50μM;30 minutes;LNCaP and PC3 cells) treatment shows a dose-dependent phosphorylation of the two major AMPK targets Acetyl-CoA Carboxylase (ACC) on Ser79 and of Raptor on Ser792.And also increases Thr172 phosphorylation on the AMPK α subunit. Cell Viability Assay |
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Cell Line: |
LNCaP and PC3 cells |
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Concentration: |
0μM,1μM,5μM,10μM,25μM,50μM |
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Incubation Time: |
4 days |
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Result: |
A dose-dependent decrease in cell numberconcomitant to the activation of AMPK signaling was observed. |
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Cell Cycle Analysis |
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Cell Line: |
LNCaP and CRPC cells |
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Concentration: |
25μM |
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Incubation Time: |
24 hours |
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Result: |
Induced a significant enrichement in the G2/M population in both androgen sensitive and CRPC cell models. |
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Apoptosis Analysis |
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Cell Line: |
LNCaP, PC3, C4-4, C4-2B, CL1and 22RV1cells |
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Concentration: |
0μM,10μM,25μM,50μM |
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Incubation Time: |
24 hours |
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Result: |
Induced reduction of anti-apoptotic Mcl-1 in concert with accumulation of the proapoptotic BH3-only protein Puma in all PCa cells. |
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Western Blot Analysis |
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Cell Line: |
LNCaP and PC3 cells |
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Concentration: |
0μM,0.25μM,0.5μM,1μM,5μM,25μM,50μM |
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Incubation Time: |
30 minutes |
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Result: |
Observed a dose-dependent phosphorylation of the two major AMPK targets Acetyl-CoA Carboxylase (ACC) on Ser79 and of Raptor on Ser792.A corresponding increase in Thr172 phosphorylation on the AMPK α subunit was also observed. |
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体内研究 |
MT 63-78 (30mg/kg;intraperitoneal injection;daily;for 14 days;C57 BL/6 male mice) treatment leads to a 33% inhibition of tumor growth. |
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Animal Model: |
C57 BL/6 male mice bearing LNCaP tumors |
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Dosage: |
30mg/kg |
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Administration: |
Intraperitoneal injection;daily;for 14 days |
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Result: |
Led to a 33% inhibition of tumor growth. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )