中文名称: | MMAF hydrochloride | ||||
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英文名称: | MMAF hydrochloride | ||||
别名: | Monomethylauristatin F hydrochloride | ||||
CAS No: | 1415246-68-2 | 分子式: | C39H66ClN5O8 | 分子量: | 768.42 |
CAS No: | 1415246-68-2 | ||||
分子式: | C39H66ClN5O8 | ||||
分子量: | 768.42 |
基本信息
产品编号:M10684 |
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产品名称:MMAF hydrochloride |
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CAS: |
1415246-68-2 |
储存条件 |
粉末 |
2-8℃
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四年 |
分子式: |
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分子量: |
768.42 |
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化学名: |
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Solubility (25°C) |
体外 |
DMSO |
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Ethanol |
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Water |
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体内(现配现用) |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
1.3014mL |
6.5069mL |
13.0137mL |
5mM |
0.2603mL |
1.3014mL |
2.6027mL |
10mM |
0.1301mL |
0.6507mL |
1.3014mL |
生物活性
产品描述 |
一种有效的微管蛋白聚合抑制剂,用作抗肿瘤药物。 |
靶点/IC50 |
Auristatin |
体外研究 |
MMAF inhibits anaplastic large cell lymphoma Karpas 299, breast carcinoma H3396,renal cell carcinoma 786-O and Caki-1 cells with IC50s of 119, 105, 257 and 200nM in vitro cytotoxicity assay. |
体内研究 |
The maximum tolerated dose in mice of MMAF (>16mg/kg) is much higher than MMAE (1mg/kg). cAC10-L1-MMAF4 has an MTD of 50mg/kg in mice and 15mg/kg in rats. The corresponding cAC10-L4-MMAF4 ADC was much less toxic, having MTDs in mice and rats of >150mg/ kg and 90 mg/kg in rats, respectively. |
推荐实验方法(仅供参考)
细胞实验: |
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Cells are treated with serial dilutions of test molecules and incubated 4-6 days depending on cell line. Assessment of cellular growth and data reduction to generate IC50 values is done using Alamar Blue dye reduction assay. |
动物实验: |
Mice: When subcutaneous Karpas 299 tumor size reaches 300mm3,three animals per group receives one injection of 10mg antibody component/kg body weight of either cAC10-L1-MMAF4 or cBR96-L1-MMAF4 intravenously. Tumors are then removed and placed in optimal cutting temperature compound, and 5μm-thin frozen tissue sections are stained using immunohistochemistry evaluation. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )