中文名称: | MN-64 | ||||
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英文名称: | MN-64 | ||||
别名: | PARP MN64;2-[4-(1-Methylethyl)phenyl]-4H-1-benzopyran-4-one | ||||
CAS No: | 92831-11-3 | 分子式: | C18H16O2 | 分子量: | 264.32 |
CAS No: | 92831-11-3 | ||||
分子式: | C18H16O2 | ||||
分子量: | 264.32 |
基本信息
产品编号:M10332 |
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产品名称:MN-64 |
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CAS: |
92831-11-3 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
两年 |
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分子量 |
264.32 |
-20℃ |
一个月 |
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化学名: |
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Solubility (25°C) |
体外 |
DMSO |
53mg/mL (200.51mM) |
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Ethanol |
53mg/mL (200.51mM) |
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Water |
Insoluble |
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体内 |
现配现用 |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
3.7833mL |
18.9165mL |
37.8329mL |
5mM |
0.7567mL |
3.7833mL |
7.5666mL |
10mM |
0.3783mL |
1.8916mL |
3.7833mL |
50mM |
0.0757mL |
0.3783mL |
0.7567mL |
生物活性
产品描述 |
一种高效的选择性的Tankyrase 1和2抑制剂,IC50分别为6和72nm。 |
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靶点/IC50 |
TNKS1 6nM (IC50) |
TNKS1 6nM (IC50) |
ARTD1 19.1μM (IC50) |
ARTD2 39.4μM (IC50) |
体外研究 |
MN-64 is a potent tankyrase 1 inhibitor, with IC50s of 6nM, 72nM, 19.1μM, 39.4μM for TNKS1, TNKS2, ARTD1 and ARTD2, respectively. MN-64 effectively inhibits Wnt/β-catenin at 1μM, and blocks STF luciferase activity at 200nM. |
推荐实验方法(仅供参考)
激酶实验: |
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Kinase Assay |
Inhibitory potency of compounds on Tankyrase-1 enzymatic activity is evaluated using a Scintillation Proximity Assay (SPA). The assay is designed to measure compound inhibition of Tankyrase-1 autoPARsylation (Tankyrase-1 is both enzyme and substrate in this assay). Truncated recombinant human Tankyrase-1 protein (amino acids E1023-T1327) is purified from SF9 cells. The assay is conducted using 0.11μM of Tankyrase-1 protein and 3μM nicotinamide adenine dinucleotide (NAD+ , 2.12μM 3H-NAD+ with a specific radioactivity of 1690 Ci/mol, 0.88 μM biotin- NAD+), in pH 7.5 Tris buffer (60mM Tris, 1mM DTT, 0.01% (v/v) Tween-20®, 2.5mM MgCl2, 0.3mg/mL BSA). For IC50 determination, 10mM DMSO stock solution of a compound (MN-64) is sequentially diluted by two-fold in DMSO, and aliquots of the diluted solutions are transferred to 384-well assay plates and mixed with Tankyrase-1 solution. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )