中文名称: | LCH-7749944 | ||||
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英文名称: | LCH-7749944 | ||||
别名: | GNF-PF-2356 | ||||
CAS No: | 796888-12-5 | 分子式: | C20H22N4O2 | 分子量: | 350.41 |
CAS No: | 796888-12-5 | ||||
分子式: | C20H22N4O2 | ||||
分子量: | 350.41 |
基本信息
产品编号:L10331 |
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产品名称:LCH-7749944 |
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CAS: |
796888-12-5 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
350.41 |
-20℃ |
一个月 |
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化学名: |
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Solubility (25°C) |
体外 |
DMSO |
70mg/mL (199.76mM) |
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Ethanol |
70mg/mL (199.76mM) |
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Water |
Insoluble |
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体内(现配现用) |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
2.8538mL |
14.2690mL |
28.5380mL |
5mM |
0.5708mL |
2.8538mL |
5.7076mL |
10mM |
0.2854mL |
1.4269mL |
2.8538mL |
50mM |
0.0571mL |
0.2854mL |
0.5708mL |
生物活性
产品描述 |
一种有效的 PAK4 抑制剂,IC50 为 14.93μM。 |
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靶点/IC50 |
PAK4 |
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14.93μM |
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体外研究 |
LCH-7749944 (GNF-PF-2356; 5-50μM; 24 hours) inhibits the proliferation of MKN-1,BGC823, SGC7901 and MGC803 cells in a concentration dependent manner.LCH-7749944 (5-20μM; 12-48 hours) induces apoptosis of SGC7901 cells.LCH-7749944 (5-20μM;12-48 hours) prominently induces a dose-dependent increase in the percentage of cells in G1 phase and decrease in S phase.LCH-7749944 (5-30μM; 24 hours) dramatically decreases levels of phosphoPAK4,phospho-c-Src,phospho-EGFR and cyclin D1 protein expression in a dose-dependent manner. Cell Proliferation Assay |
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Cell Line: |
MKN-1, BGC823, SGC7901 and MGC803 human gastric cancer cells |
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Concentration: |
5, 10, 15, 20, 25, 30, 35, 40, 45, 50μM |
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Incubation Time: |
24 hours |
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Result: |
Inhibited the proliferation of MKN-1, BGC823, SGC7901 and MGC803 cells in a concentration dependent manner. |
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Apoptosis Analysis |
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Cell Line |
SGC7901 cells |
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Concentration: |
5, 10, 20μM |
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Incubation Time: |
12, 24, 48 hours |
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Result: |
Induced apoptosis of SGC7901 cells. |
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Cell Cycle Analysis |
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Cell Line: |
SGC7901 cells |
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Concentration: |
5, 10, 20μM |
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Incubation Time: |
12, 24, 48 hours |
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Result: |
Prominently induced a dose-dependent increase in the percentage of cells in G1 phase and decrease in S phase. |
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Western Blot Analysis |
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Cell Line: |
SGC7901 cells |
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Concentration: |
5, 10, 20, 30μM |
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Incubation Time: |
24 hours |
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Result: |
Dramatically decreased levels of phosphoPAK4, phospho-c-Src, phospho-EGFR and cyclin D1 protein expression in a dose-dependent manner. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )