中文名称: | 昆布多糖 来源于褐藻类 | ||||
---|---|---|---|---|---|
英文名称: | Laminaran from Eisenia Bicyclis | ||||
别名: | 昆布多糖 (3R,4S,5R,6R)-4-(((3R,4S,5R,6R)-3,5-dihydroxy-6-(hydroxymethyl)-4-(((3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)tetrahydro-2H-pyran-2-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-2,3,5-triol | ||||
CAS No: | 9008-22-4 | 分子式: | C18H32O16 | 分子量: | 504.437088012695 |
CAS No: | 9008-22-4 | ||||
分子式: | C18H32O16 | ||||
分子量: | 504.437088012695 | ||||
MDL: | MFCD00466918 | EINEC: | 232-712-4 | ||
EINEC: | 232-712-4 |
基本信息
产品编号: |
L10054 |
||||
产品名称: |
Laminaran from Eisenia Bicyclis |
||||
CAS: |
9008-22-4 |
储存条件 |
粉末 |
室温 |
四年 |
|
|
||||
分子式: |
溶于液体 |
-80℃ |
六个月 |
||
分子量 |
504.44 |
-20℃ |
一个月 |
||
化学名: |
(3R,4S,5R,6R)-4-(((3R,4S,5R,6R)-3,5-dihydroxy-6-(hydroxymethyl)-4-(((3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)tetrahydro-2H-pyran-2-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-2,3,5-triol |
||||
Solubility (25°C): |
|||||
体外:
|
DMSO |
100mg/mL (Need ultrasonic) |
|||
Ethanol |
|
||||
Water |
100mg/mL (Need ultrasonic) |
||||
1.请依序添加每种溶剂:10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 2.5mg/mL (InfinitymM); Clear solution |
|||||
此⽅案可获得 ≥ 2.5mg/mL (InfinitymM,饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例,取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中,混合均匀。 |
|||||
2.请依序添加每种溶剂:10% DMSO→90% corn oil Solubility: ≥ 2.5mg/mL (InfinitymM); Clear solution |
|||||
此⽅案可获得 ≥ 2.5mg/mL (InfinitymM,饱和度未知) 的澄清溶液,此⽅案不适⽤于实验周期在半个⽉以上的实验。 以 1mL ⼯作液为例,取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中,混合均匀。 |
|||||
<1mg/ml表示微溶或不溶。 |
|||||
普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
|||||
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
生物活性
产品描述 |
一种在褐藻中发现的葡萄糖多糖。 |
|
体外研究 |
Laminaran (100-800µg/mL; 24 hours) is not cytotoxic against normal epidermal cells JB6 Cl41 and human melanoma cells SK-MEL-28, the percentage of inhibition of living cells number is less than 15 % at concentrations range up to 800µg/mL after 24 h of treatment Laminaran (200µg/mL; 24-72 hours)does not cause any growth inhibition of SK-MEL-28 cells after 24 and 48 h of treatment, but decreases cells proliferation by 36 % after 72 h of treatment. Laminaran (25-50µg/mL; 24 hours) at low concentration does not influence the phosphorylation of c-Raf (Ser259), ERK1/2 (Tyr202/Tyr204), and MEK1/2 (Ser 221) kinases as well as total expression level of investigated proteins. But decreases pMEK, p-ERK1/2 at 50µg/mL Cell Viability Assay |
|
|
Cell Line: |
JB6 Cl41 and SK-MEL-28 cells |
Concentration: |
100, 200, 400, and 800µg/mL |
|
Incubation Time: |
24 hours |
|
Result: |
Showed no cytotoxic against normal epidermal cells JB6 Cl41 and human melanoma cells SK-MEL-28. |
|
Cell Proliferation Assay |
||
Cell Line: |
SK-MEL-28 cells |
|
Concentration: |
200µg/mL |
|
Incubation Time: |
24 hours |
|
Result: |
Decreased cell proliferation at 72 hours. |
|
Western Blot Analysis |
||
Cell Line: |
SK-MEL-28 cells |
|
Concentration: |
25µg/mL; 50µg/mL |
|
Incubation Time: |
24 hours |
|
Result: |
Inhibited phosphorylation of c-Raf, MEK1/2, and ERK1/2 kinases. |
|
体内研究 |
Laminaran (intravenous injection; 12.5, 25, and 50mg/kg; 21 days) and OVA (50µg) combination significanly decreases the tumor masses when it compares with the PBS-, OVA-, and laminarin-treated mice |
|
Animal Model: |
C57BL/6 mice injected s.c. with B16-OVA cells |
|
Dosage: |
12.5, 25, and 50mg/kg; 21 days |
|
Administration: |
Intravenous injection |
|
Result: |
Prevented B16-OVA tumor growth by inducing Ag-specific immune responses. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )