| 中文名称: | Kynurenic acid sodium | ||||
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| 英文名称: | Kynurenic acid sodium | ||||
| CAS No: | 2439-02-3 | 分子式: | C10H6NNaO3 | 分子量: | 211.15 |
| CAS No: | 2439-02-3 | ||||
| 分子式: | C10H6NNaO3 | ||||
| 分子量: | 211.15 | ||||
基本信息
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产品编号： |
K10087 |
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产品名称： |
Kynurenic acid sodium |
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CAS: |
2439-02-3 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量： |
211.15 |
-20℃ |
1个月 |
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化学名： |
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Solubility (25°C)： |
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体外:
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DMSO |
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Ethanol |
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Water |
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体内(现配现用): |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
4.7360mL |
23.6798mL |
47.3597mL |
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5mM |
0.9472mL |
4.7360mL |
9.4719mL |
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10mM |
0.4736mL |
2.3680mL |
4.7360mL |
生物活性
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产品描述 |
一种内源性色氨酸代谢物，是针对靶点 NMDA,glutamate,α7 nicotinic acetylcholine receptor的光谱性拮抗剂。Kynurenic acid sodium也是 GPR35/CXCR8的激动剂。 |
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靶点 |
Target:GPR35,NMDA,glutamate,glutamate,α7 nicotinic acetylcholine |
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体外研究 |
GPR35 functions as a receptor for the kynurenine pathway intermediate kynurenic acid.Kynurenic acid elicits calcium mobilization and inositol phosphate production in a GPR35-dependent manner in the presence of G qi/o chimeric G proteins.Kynurenic acid stimulates [35S]guanosine 5′-O-(3-thiotriphosphate) binding in GPR35-expressing cells,an effect abolished by pertussis toxin treatment.Kynurenic acid also induces the internalization of GPR35.KYNA’s neuroinhibitory qualities and its neuroprotective and anticonvulsant effects are discovered using concentrations of the compound in the millimolar range.This,as well as the low affinity of KYNA at each of the three ionotropic glutamate receptors responsible for these effects [NMDA,alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainate],together with the realization that KYNA concentrations in the mammalian brain are in the sub-micromolar range,suggested that other receptors might serve as targets of endogenous Kynurenic acid.Kynurenic acid,with a shallower inhibition curve and noncompetitively,antagonizes α7nAChRs on cultured hippocampal neurons with an IC50 in the low micromolar range. |
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体内研究 |
Kynurenic acid affects the activity of leukocytes in the peripheral blood of mice,although the lowest one (2.5mg/L) has the most profound influence in contrast to the highest one (250mg/L),which produces the weakest effect.The lowest Kynurenic acid dose stimulates the proliferative response of T lymphocytes (p<0.05),after 7 and 28 days of administering the acid to the animals. |
推荐实验方法(仅供参考)
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激酶实验： |
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CHO-GPR35 stable cells are pretreated with or without pertussis toxin (100ng/mL) for 16h before harvesting.Cells are resuspended and homogenized in 10mM Tris-HCl (pH 7.4),1mM EDTA followed by centrifugation at 1000×g for 10 min at 4℃ to remove nuclei and cellular debris.Membrane fractions are collected by spinning the supernatant at 38,000×g for 30 min and resuspended in 20mM HEPES (pH 7.5) and 5mM MgCl2. 25μg of membranes is incubated at room temperature for 1h in assay buffer (20mM HEPES,5m MMgCl2,0.1% bovine serum albumin (pH 7.5)) containing 3μM GDP and 0.1nM[35S]GTPγS in the absence or presence of kynurenic acid.Reactions are terminated by vacuum filtration through GF/B filters,and the retained radioactivities are quantified on liquid scintillation counter. |
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动物实验: |
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Mouse:The experiment is performed on 160 male BALB/c mice,aged 10-12 weeks,with body weight of 22-26g.The animals are maintained on a 12-h light/dark cycle at controlled temperature (20±1℃) and supplied with rodent chow and water ad libitum throughout the experiment.Mice are divided randomLy into four equal groups:control group (0) not receiving the Kynurenic acid,and three experimental groups administered the Kynurenic acid solution in drinking water at concentrations of 2.5,25 or 250mg/L.After 3,7,14 and 28 consecutive days of administration of the Kynurenic acid solution,10 individuals from each group are sacrificed.The animals are anesthetized by inhalation of Aerrane and their blood is collected by heart puncture.Blood collected from five individuals of each group is used for the MTT assay, and from the next five for the flow cytometry. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
