山奈酚 - CAS:520-18-3 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	山奈酚
英文名称:	Kaempferol
别名:	山奈酚 Kaempferol
CAS No:	520-18-3	分子式:	C₁₅H₁₀O₆	分子量:	286.24
CAS No:	520-18-3
分子式:	C₁₅H₁₀O₆
分子量:	286.24
MDL:	MFCD00016938	EINEC:	208-287-6
EINEC:	208-287-6

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K10019 山奈酚 ≥98%(HPLC),分析标准品 (psaitong)

相关产品

包装规格：

20mg in glass bottle

产品描述：

基本信息

产品编号：	K10019
产品名称：	Kaempferol
CAS:	520-18-3	储存条件	粉末	室温	四年

分子式：	C15H10O6		溶于液体	-80℃	两年
分子量	286.24			-20℃	一个月
化学名：	3,5,7-Trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one
Solubility (25°C)：
体外:	DMSO	27mg/mL warmed with 50ºC water bath (94.32mM)
	Ethanol	28mg/mL (97.82mM)
	Water	Insoluble
体内(现配现用):	1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2mg/mL (6.99mM); Clear solution
	此⽅案可获得 ≥ 2mg/mL (6.99mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 20.0mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。
	2.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 2mg/mL (6.99mM); Clear solution
	此⽅案可获得 ≥ 2mg/mL (6.99mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 20.0mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。
	3.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2mg/mL (6.99mM); Clear solution
	此⽅案可获得 ≥ 2mg/mL (6.99mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以 1mL ⼯作液为例，取 100μL 20.0mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	3.4937mL	17.4685mL	34.9369mL
5mM	0.6987mL	3.4937mL	6.9874mL
10mM	0.3494mL	1.7468mL	3.4937mL
50mM	0.0699mL	0.3494mL	0.6987mL

生物活性

产品描述	Kaempferol (NSC 407289, Robigenin)是一种黄酮，是ERRα和ERRγ的反向激动剂。抑制topoisomerase I催化的DNA再连接和fatty acid synthase的活性。
靶点	ERRα	ERRγ	Topo I	fatty acid synthase
体外研究	Kaempferol also has anti-inflammatory effects via inhibition of interleukin-4 and cyclo-oxygenase 2 expression by suppressing Src kinase and downregulating the NFκB pathway. Kaempferol is also effective in inhibiting angiogenesis and inducing apoptosis in ovarian cancer cells. Kaempferol is a natural flavonoid that is widely distributed in fruits and vegetables, and prospective studies revealed that over decades, consumption of Kaempferol dramatically and significantly reduces the risk of ovarian cancer in American female nurses. After a 24-hour treatment, Kaempferol causes a significant and concentration-dependent inhibition of proliferation in all 3 ovarian cancer cells tested. This inhibition is observed at 40μM or higher concentrations of treatment. Kaempferol is a flavonoid which is abundant in a variety of plant derived food and leaves used in traditional medicines. Kaempferol significantly inhibits NADPH oxidase activity. Kaempferol decrease reactive oxygen species (ROS) by directly bound NADPH oxidase. Kaempferol prevents Ang II-induced sinus nodal cell death by lowering CAMKII oxidization.10-20μM Kaempferol dose-dependently suppresses its release in sensitized RBL-2H3 cells. When 10-20μM Kaempferol is supplemented to DNP-BSA-challenged RBL-2H3 cells for 15 min, the activation of Syk and PLC γ is highly attenuated. When ≥10μM Kaempferol is added to DNP-BSA-challenged RBL-2H3 cells for 60 min, the COX2 induction is reduced
体内研究	The COX2 induction is confirmed in the airways of BSA-challenged BALB/c mice. There is lack of COX2 in airways of untreated control mice observed. The BSA inhalation to mice led to enhanced COX2 induction (dark brown staining) in mouse airway, which is reversed by oral administration of Kaempferol. In BSA-challenged mice, there is a marked goblet cell hyperplasia and epithelial thickening observed. When 20mg/kg Kaempferol is supplemented to BSA-challenged mice, the epithelial thickening completely disappeared

推荐实验方法(仅供参考)

Kinase Assay	Right atria or sinus nodal cells are homogenized in lysis buffer consisting of (50mM Tris-HCl pH 7.5, 100mM KCl, 1mM ethylenediamine tetraacetic acid, 1mM ethylene glycol tetraacetic acid, 1mM dithiothreitol, 0.1mM phenylmethylsulfonyl fluoride, 0.5mM Benzamidine, 20mg/L Leupeptin, 20mM sodium pyrophosphate, 50mM NaF, and 50mM sodium βglycerophosphate), and total protein content is determined by the Bradford assay. Caspase-3 activity is determined by EnzChek Caspase-3 Assay Kit

Cell Assay	Ovarian cancer cells are seeded in 96-well plates at 2000 cells/well and incubated overnight before treatment with 0-160μM Kaempferol for 24 hours in triplicates. The medium is removed, and the plates are freeze-thawed to lyse cells. Each well is added with 200μL 1× CyQUANT cell lysis buffer containing 5x SYBR Green I and incubated at room temperature (RT) for 5 minutes. The reaction (50μL) is transferred to PCR strip tubes and the fluorescent signal is measured at 90°C with a realtime Chromo4 PCR instrument. To ensure that cell proliferation assays are performed within a linear range of cell numbers, a standard curve is generated by seeding different amount of OVCAR-3 cells (based on counting with a hemacytometer) in a 96-well plate, and measuring genomic DNA abundance after overnight incubation. Three independent experiments are performed and data is pooled for statistical analysis

Animal Administration	Mice Three-week-old male BALB/c mice are randomly assigned to the four treatment groups as follows (n=8 per group). (1) PBSsensitized mice; (2) BSA-sensitized mice; (3) BSA-sensitized and 10mg/kg Kaempferol-administered mice; and (4) BSAsensitized and 20mg/kg Kaempferol-administered mice. Mice are given a commercial mouse chow diet containing 20.5% protein, 3.5% fat, 8% fiber, 8% ash, and 0.5% phosphorus and are allowed access to food and water ad libitum. The mice are kept under a 12 h light and dark cycle at 23±1°C with 50%±5% relative humidity in specific pathogen-free conditions. Mice are allowed to become accustomed to their surroundings for one week before starting the allergic experiments. Sensitization of all experimental mice is carried out by subcutaneous injection with 20μg BSA in 30μL PBS and 50μL Imject Alum on days 0 and 14. The control mice are injected with a combination of 50μL PBS and 50μL Imject Alum without BSA. On days 28, 29, and 30, only the experimental mice sensitized to BSA are subject to inhalation of 5% BSA, while control mice are challenged with 5% PBS for 20 min in a plastic chamber connected to a Medel aerosol nebulizer. All mice are sacrificed 24 h after the last challenge. Whole blood samples are directly used to measure the contents of eosinophils, basophils and neutrophils. The right lung is stored in 4% paraformaldehyde until use.