| 中文名称: | 依鲁替尼-生物素 | ||||
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| 英文名称: | Ibrutinib-biotin | ||||
| 别名: | 依鲁替尼-生物素 Ibrutinib-biotin | ||||
| CAS No: | 1599432-18-4 | 分子式: | C56H80N12O9S | 分子量: | 1097.37 |
| CAS No: | 1599432-18-4 | ||||
| 分子式: | C56H80N12O9S | ||||
| 分子量: | 1097.37 | ||||
基本信息
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产品编号：I10417 |
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产品名称：Ibrutinib-biotin |
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CAS: |
1599432-18-4 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
1097.37 |
-20℃ |
一个月 |
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化学名： |
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Solubility (25°C) |
体外 |
DMSO |
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Ethanol |
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Water |
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体内（现配现用） |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
0.9113mL |
4.5563mL |
9.1127mL |
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5mM |
0.1823mL |
0.9113mL |
1.8225mL |
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10mM |
0.0911mL |
0.4556mL |
0.9113mL |
生物活性
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产品描述 |
一种由 Ibrutinib 通过长链接头连接到生物素上组成的探针，具体可参考 WO2014059368A1 中 Compound 1-5，抑制 BTK，IC50 值为 0.755-1.02nM。 |
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靶点/IC50 |
IC50:0.755-1.02nM (BTK),1.71-2.29nM (TEC),3.66-4.27nM (BLK), 4.42-5.57nM (BMX), 5.99-6.92nM (LCK), 33.8-36.3nM (Src), 0.187-0.198μM (ITK),5.34-4.87μM (JAK3) |
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体外研究 |
Ibrutinib-biotin (Compound 1-5) is a probe that consists of Ibrutinib linked to biotin via a long chain linker. Ibrutinib is a TEC family kinase inhibitor. The TEC kinase family is composed of five members, TEC, BTK (Bruton's Tyrosine Kinase), ITK (interleukin-2-inducible T-cell kinase)/EMT/TSK, BMX and TXK/RLK. The TEC family kinases participate in phosphotyrosinemediated and phospholipid-mediated signaling systems. Many TEC family proteins are abundantly expressed in hematopoietic tissues, and play important roles in the growth and differentiation processes of blood cells. |
推荐实验方法(仅供参考)
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激酶实验： |
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In this study, assay formats are tested for sensitivity, specificity, range, and to determine most suitable anti BTK Antibody.An aliquot of DOHH2 cell lysate (1mg/mL) is inhibited with 1μM Ibrutinib then labeled with probe (1μM). Negative controls: Untreated DOHH2 and Jurkat cell lysates (lmg/mL) are labeled with probe (1μM) and Untreated DOHH2 cell lysate.Standard Streptavidin plate (5-pack); Read Buffer T (50mL), SULFO TAG Goat Anti-mouse (50μg); SULFO TAG Goat Antirabbit (50μg); SULFO TAG streptavidin (50ug); MSD Standard Plates; MSD Blocker A; Protease inhibitor cocktail; Positive Control lysates from BTK expressing cell line (DOHH2); Negative Control lysates; Ibrutinib (PCI); probe compound 1-5 (biotinylated probe). |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
