INH6 - CAS:1001753-24-7 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	INH6
英文名称:	INH6
别名:	N-(4-异丙叉噻唑-2-基)苯甲酰胺;N-[4-(2,4,6-三甲基苯基)-2-噻唑基]苯甲酰胺 N-(4-mesitylthiazol-2-yl)benzamide;INH6;N-[4-(2,4,6-trimethylphenyl)thiazol-2-yl]benzamide;N-(4-Mesitylthiazol-2-yl)benzaMide;N-[4-(2,4,6-trimethylphenyl)-2-thiazolyl]-benzamide
CAS No:	1001753-24-7	分子式:	C₁₉H₁₈N₂Os	分子量:	322.42
CAS No:	1001753-24-7
分子式:	C₁₉H₁₈N₂Os
分子量:	322.42

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I10020 INH6 ≥98%(HPLC) (psaitong)

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包装规格：

5mg 25mg 100mg in glass bottle

溶解性：

溶于DMSO（64mg/mL ）和乙醇

产品描述：

基本信息

产品编号：I10020
产品名称：INH6
CAS:	1001753-24-7	储存条件	粉末	-20℃	四年

分子式：	C19H18N2Os		溶于液体	-80℃	六个月
分子量：	322.42			-20℃	一个月
化学名：
Solubility (25°C)	体外	DMSO	64mg/mL warmed with 50ºC water bath (198.49mM)
		Ethanol	11mg/mL warmed with 50ºC water bath (34.11mM)
		Water	Insoluble
	体内（现配现用）
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	3.1015mL	15.5077mL	31.0154mL
5mM	0.6203mL	3.1015mL	6.2031mL
10mM	0.3102mL	1.5508mL	3.1015mL
50mM	0.0620mL	0.3102mL	0.6203mL

生物活性

产品描述

一种强效的Hec1抑制剂，破坏Hec1/Nek2相互作用，导致染色体排列异常，并且在MDA-MB231，MDA-MB468，HeLa 和K562细胞系中表现出有效的细胞杀伤活性，IC50分别为1.7，2.1，2.4和 2.5μM。

靶点/IC50

2.4μM (HeLa),1.7μM (MB231),2.1μM (MB468),2.5μM (K562)

体外研究

Hec1 is an oncogene overly expressed in many human cancers. Small molecule INH (Inhibitor of Nek2/Hec1) targeting the Hec1 and its regulator, Nek2, in the mitotic pathway is identified to inactivate Hec1/Nek2 function mediated by protein degradation that subsequently leads to chromosome mis-segregation and cell death. INH6 treated cells exhibit increased mitotic population with multipolar spindle configurations. An increased rate of chromosome misalignment is detected upon treatment with INH6 of HeLa cells expressing the chromosome marker protein H2B-GFP. INH6 treated cells shows progressive morphological changes characteristic of dying cells (e.g., membrane bubbling), which is further confirmed by cell cycle profiling with FACS analysis. Approximately 20% of INH6 treated cells are apoptotic 72 hrs after treatment.

推荐实验方法(仅供参考)

细胞实验:

Standard XTT assays with a four-day drug treatment procedure were performed to measure the dose-dependent cytotoxicity of INH analogs in cultured cells. Triplicate sets were measured and compiled for final data presentation. Cells were plated on 96-well dishes one day before the drug treatment, followed by drug treatment (2.5μM INH6) on day 2 and XTT assay on day 5 after drug addition. The absorption at 595nm was measured with a plate reader and converted to cell survival percentages in comparison to mock treated groups.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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