ITD-1 - CAS:1099644-42-4 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	ITD-1 促销
英文名称:	ITD 1
别名:	ITD-1 4-[1,1'-Biphenyl]-4-yl-1,4,5,6,7,8-hexahydro-2,7,7-trimethyl-5-oxo-3-quinolinecarboxylic acid ethyl ester
CAS No:	1099644-42-4	分子式:	C₂₉H₃₂N₆O₄S	分子量:	560.67
CAS No:	1099644-42-4
分子式:	C₂₉H₃₂N₆O₄S
分子量:	560.67

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF) 点击下载技术规格说明书(PDF)

I10015 ITD-1 ≥98% (HPLC) (psaitong)

相关产品

包装规格：

2mg 5mg 25mg 100mg in glass bottle

溶解性：

溶于DMSO（6mg/ml)

产品描述：

基本信息

产品编号：	I10015
产品名称：	ITD-1
CAS:	1099644-42-4	储存条件	粉末	-20℃	四年

分子式：	C29H32N6O4S		溶于液体	-80℃	两年
分子量	560.67			-20℃	1个月
化学名：	4-[1,1'-Biphenyl]-4-yl-1,4,5,6,7,8-hexahydro-2,7,7-trimethyl-5-oxo-3-quinolinecarboxylic acid ethyl ester
Solubility (25°C)：
体外:	DMSO	83 mg/mL (199.74mM)
	Water	Insoluble
	Ethanol	Insoluble
体内(现配现用):	1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.5 mg/mL (6.02mM); Clear solution
	此方案可获得 ≥ 2.5 mg/mL (6.02mM，饱和度未知) 的澄清溶液。以 1mL 工作液为例，取 100μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加入50μL Tween-80，混合均匀；然后继续加入 450μL生理盐水定容至 1mL。
	2.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2.5 mg/mL (6.02mM); Clear solution
	此方案可获得 ≥ 2.5 mg/mL (6.02 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。以 1mL 工作液为例，取 100μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900μL玉米油中，混合均匀。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.4066mL	12.0331mL	24.0662mL
5mM	0.4813mL	2.4066mL	4.8132mL
10mM	0.2407mL	1.2033mL	2.4066mL
50mM	0.0481mL	0.2407mL	0.4813mL

生物活性

产品描述	一种选择性TGF-β抑制剂.
靶点	IC50: 460nM (TGFβ receptor)
体外研究	ITD-1 potently blocks phosphorylation of the effector SMAD2/3 proteins induced by TGFβ2, and only minimally in response to Activin A. HEK293T cells are transfected with a Smad4 response element driving luciferase (SBE4-Luc) to test whether ITD1 blocks Activin A/Nodal and/or TGFβ signaling, which utilize the same intracellular signaling cascade through Smad4. ITD-1 strongly inhibits TGFβ2 signaling with similar efficacy (92% vs. 99% respectively), but with lower potency compared to SB431542, an ACVR1B/TGFBR1 kinase inhibitor (IC50= 850nM vs. 70nM respectively), and is a weak and partial inhibitor of Activin A signals. ITD-1 selectively enhances the differentiation of uncommitted mesoderm to cardiomyocytes, but not to vascular smooth muscle and endothelial cells. ITD-1 reveals an unexpected role for TGFβ signaling in controlling cardiomyocyte differentiation from multipotent cardiovascular precursors

推荐实验方法(仅供参考)

Cell Assay

For the Smad4-Response element (SBE4) assay, 293T cells are grown in Phenol Free DMEM-high glucose supplemented with 1% FBS. About 30000 cells/cm2 are reverse transfected onto white 384-well cell-culture plates with 10ng of SBE4-Lux and CMV-Renilla-Lux using Lipofectamine 2000. Cells are allowed to adhere for at least 12 hours and induced with either TGFβ2 (15ng/mL), Activin A (15ng/mL). Simultaneously, ITD-1 (0.001, 0.01, 0.1, 1, and 10μM) is added to cells. SB-431542 is used as a positive control for Activin A/TGFβ-signaling inhibition. To determine luminescence levels, Dual-Glo kit is used and measured on an Envision plate reader. Firefly luminescence is normalized against renilla luciferase

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

搜索质检报告(COA)

参考文献 & 客户发表文献