| 中文名称: | 2-HBA | ||||
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| 英文名称: | 2-HBA | ||||
| 别名: | (E,E)-双(2-羟基亚苄基)乙酮 (2-HBA);4)-2-(乙酰氨基)-2-脱氧-BETA-D-吡喃葡萄糖苷;(E，E)-Bis(2-hydroxybenzylidene)acetone(2-HBA) 国华试剂 (E,E)-Bis(2-hydroxybenzylidene)acetone(2-HBA);(E,E)-Bis(2-hydroxybenzylidene)acetone (2-HBA);2-HBA;(1E,4E)-1,5-Bis(2-Hydroxyphenyl)-1,4-pentadien-3-one;HBB2;(E,E)-Bis(2-hydroxybenzylidene)acetone\n(2-HBA) | ||||
| CAS No: | 131359-24-5 | 分子式: | C17H14O3 | 分子量: | 266.29 |
| CAS No: | 131359-24-5 | ||||
| 分子式: | C17H14O3 | ||||
| 分子量: | 266.29 | ||||
基本信息
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产品编号：H10339 |
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产品名称：2-HBA |
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CAS: |
131359-24-5 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
266.29 |
-20℃ |
一个月 |
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化学名： |
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Solubility (25°C) |
体外 |
DMSO |
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Ethanol |
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Water |
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体内（现配现用） |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
3.7553mL |
18.7765mL |
37.5530mL |
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5mM |
0.7511mL |
3.7553mL |
7.5106mL |
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10mM |
0.3755mL |
1.8777mL |
3.7553mL |
生物活性
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产品描述 |
一个有效的 NAD(P)H: 醌受体氧化还原酶 1 (NQO1) 诱导剂，也可以激活半胱天冬酶-3 (caspase-3) 和半胱天冬酶-10 (caspase-10)。 |
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靶点/IC50 |
Caspase-3 Caspase-10 |
Caspase-3 Caspase-10 |
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体外研究 |
When L1210 cells are exposed to 0.6μM 2-HBA (bis(2-hydroxy-benzylidene)acetone),the specific activities of NQO1 and glutathione reductase increase by 6- and 1.5-fold, respectively. The total cellular glutathione content is also coordinately induced by 2.4-fold. In a more detailed study it is found that NQO1 is induced by 2-HBA in a concentration-dependent manner. Treatments with 2-HBA cause cell cycle arrest and apoptosis in both L1210 wild type cells and their Y8 drugresistant counterparts in a concentration-dependent manner. 2-HBA can also activate caspase-3 and caspase-10. |
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推荐实验方法(仅供参考)
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激酶实验： |
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Cells (20,000 per well) are grown for 24h in 96-well plates, then exposed to 2-HBA (bis(2-hydroxybenzylidene)acetone) for either 24h (for glutathione determination) or 48h (for determination of enzyme activities). At the end of the exposure period, cells are collected by centrifugation (1500×g for 15 min at 4℃), washed with DPBS, and finally lysed in 0.08% digitonin.An aliquot (25μL) is used for protein analysis. Activity of NQO1 is determined by the Prochaska test. |
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细胞实验: |
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After exposure to 2-HBA (bis(2-hydroxybenzylidene)acetone) for 24h, duplicate aliquots of cells (1×106) are collected by centrifugation and washed with cold DPBS. Apoptosis is determined using the Annexin-V-FLUOS assay with simultaneous determination of the necrotic fraction by the uptake of propidium iodide. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
